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| Exploring the therapeutic effect and molecular mechanism of crocin on a young mouse pneumonia model based on the MAPK/NF-κB pathway |
| Hits 460 Download times 349 Received:August 10, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.12.17 |
| Key Words
crocin;young mice;pneumonia;MAPK/NF-κB pathway |
| Author Name | Affiliation | E-mail | | DONG Lijun | School of Clinical Medicine, Weifang Medical College, Weifang 261053, China | | | ZHOU Bo | Department of Imaging, Anqiu People's Hospital, Anqiu 262199, China | | | YANG Cuiling | Department of Pediatrics, Anqiu People's Hospital, Anqiu 262199, China | | | LI Zhiyuan | School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | | ZHANG Yongfeng | Department of Pediatrics, Affiliated Hospital of Weifang Medical College, Weifang 261035, China | zy20050415@163.com |
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| Abstract
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| [Objective] To explore the therapeutic effect of crocin(CRO) on pneumonia in young mice and its relationship with mitogen activated protein kinase(MAPK)/nuclear factor-κB(NF-κB) pathway. [Methods] A young mouse pneumonia model was constructed by intraperitoneal injection of lipopolysaccharide(LPS). The mice were randomly grouped into control group,model group,DEX(positive control dexamethasone) group,CRO low-dose group,CRO medium-dose group,CRO high-dose group,and MAPK activator(Anisomycin group,CRO high-dose+Anisomycin). The wet to dry(W/D) ratio and myeloperoxidase(MPO) activity of mouse lungs were detected.Hematoxylin eosin(HE) staining was applied to observe pathological changes in lung tissue and pathological injury score was performed.Blood cell counters and Giemsa staining were applied to perform total cell,neutrophil,and white blood cell counts. Enzyme-linked immunosorbent assay(ELISA) was applied to detect levels of inflammatory cytokines in serum and alveolar lavage fluid(BALF). Kits were applied to detect malondialdehyde(MDA) and superoxide dismutase(SOD) in serum and BALF. Western blot was applied to detect the expression of MAPK/NF-κB pathway protein. [Results] Compared with the model group,the DEX group and CRO low,medium,and high dose groups showed reduced lung tissue injury,the W/D ratio,MPO activity,pathological injury score,total cells,neutrophils,white blood cell count,IL-1β,IL-6,TNF-α,MDA,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65,p-IκBα/IκBα were obviously decreased,SOD was obviously increased(P＜0.05). Anisomycin was able to reverse the therapeutic effect of high-dose CRO on pneumonia in mice(P＜0.05). [Conclusion] CRO has therapeutic effects on young pneumonia mice,and its mechanism may be related to inhibiting the MAPK/NF-κB pathway. |
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