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| Impact of resveratrol on chondrocyte apoptosis in temporomandibular joint osteoarthritis model rats by regulating SDF-1/CXCR4 pathway |
| Hits 407 Download times 292 Received:August 16, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2023.12.18 |
| Key Words
resveratrol;SDF-1/CXCR4 axis;emporomandibular joint osteoarthritis;chondrocyte;apoptosis |
| Author Name | Affiliation | | CHEN Mengmeng | Department of Stomatology, Harbin Fourth Hospital, Harbin 150026, China | | XING Chunhua | Department of Laboratory Medicine, Harbin Fourth Hospital, Harbin 150026, China |
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| Abstract
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| [Objective] To explore the impacts of resveratrol(RES) on chondrocyte apoptosis in rats with temporomandibular joint osteoarthritis(TMJOA) and its regulatory mechanism on SDF-1/CXCR4 axis in this process. [Methods] Thirty-six Wistar male rats were randomly grouped into control group,model group,CXCR4 inhibitor group(AMD3100 group),RES low dose group,RES high dose group,and RES high dose+recombinant SDF-1 group(RES high+SDF-1 group),with 6 rats in each group. Histopathological changes of cartilage were observed by hematoxylin-eosin(HE) and safranine O-solid green staining;chondrocytes were isolated and cultured;cell apoptosis was detected by flow cytometry;enzyme-linked immunosorbent assay(ELISA) was applied to detect the levels of nitric oxide synthase(iNOS),nitric oxide(NO) and cytochrome C(Cyt C) in cells;Western blot was applied to detect the expression of caspase-9,Bcl-2,Bax,SDF-1,CXCR4 proteins in chondrocytes. [Results] Compared with the control group,the cartilage tissue structure of the model group was disordered and the number of cells was reduced. The staining of cartilage was obviously reduced. The injury score,cell apoptosis rate,iNOS,NO,Cyt C levels and the expression of caspase-9,Bax,SDF-1,CXCR4 proteins increased. The expression of Bcl-2protein decreased(P＜0.05). Compared with the model group,the structure level of cartilage tissue in AMD3100 group and RES low and high dose groups were gradually obvious. The number of cells increased,and the staining of cartilage increased. The injury score,apoptosis rate,iNOS,NO,Cyt C levels and the expression of caspase-9,Bax,SDF-1,CXCR4 proteins decreased,and the expression of Bcl-2 protein increased(P＜0.05). Further addition of recombinant protein SDF-1 showed that the increased expression of SDF-1 reversed the inhibitory effects of high-dose RES on signal pathway and chondrocyte apoptosis(P＜0.05). [Conclusion] RES can inhibit chondrocyte apoptosis in TMJOA rats by blocking SDF-1/CXCR4 signal pathway. |
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