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| Study on the mechanism of regulating RhoA/ROCK1 pathway by acupuncture at Renying Point to improve blood pressure and vascular injury in spontaneously hypertensive rats |
| Hits 530 Download times 339 Received:September 26, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.01.14 |
| Key Words
Renying Point;acupuncture;blood pressure;ET-1;Ang Ⅱ;PDGF-AA;MMP2;RhoA/ROCK1 pathway |
| Author Name | Affiliation | E-mail | | ZHOU Shiyuan | Department of Acupuncture, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | | | YANG Yongke | Department of Psychiatry, Ningbo Beilun District People's Hospital, Ningbo 315800, China | | | XIONG Feiran | Department of Traditional Chinese Medicine, Hebei Sanhe Traditional Chinese Medicine Hospital, Langfang 065200, China | | | LU Yan | Department of Traditional Chinese Medicine, Beichen District Shuanghuantun Street Community Health Service Center, Tianjin 300134, China | | | ZHAO Yiwen | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | LIU Jiayan | Department of Acupuncture, Xiqing District Zhongbei Community Health Service Center, Tianjin 300111, China | | | SHI Huiyan | Department of Acupuncture, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | maginhuiyan@163.com | | SHI Xuemin | Department of Acupuncture, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China | tjdrshi@msn.com |
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| Abstract
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| [Objective] To explore the effect and mechanism of acupuncture at Renying Point on blood pressure and vascular injury in spontaneously hypertensive rats(SHR) by regulating RhoA/ROCK1 signaling pathway. [Methods] The 11 week-old male spontaneously hypertensive rats(SHR) and Wistar rats were randomly divided into four groups:SHR group,acupuncture group,medicine group and Wistar group. The medicine group was given the irbesartan by gavage,the acupuncture group was treated with acupuncture on the acupoints of Renying(ST-9). Observe the changes in blood pressure,detect the expression levels of Ang Ⅱ and ROCK1 using ELISA,detect the mRNA and protein expression levels of ET-1 and PDGF-AA using RT-PCR and Western Blot,and detect the localization and expression levels of MMP2 and RhoA in carotid artery tissue using immunofluorescence. [Results] 1)Compared with the model group,the mean arterial pressure of the medicine group and the acupuncture group at 3 weeks and 6 weeks of treatment was significantly reduced.(P＜0.05). 2)Compared with model group,mRNA and protein expressions of ET-1 and PDGF-AA in Western medicine group and acupuncture group were significantly reduced after 6 weeks of treatment(P＜0.05). 3)Compared with model group,the protein expressions of RhoA,AngⅡ and ROCK1 in western medicine group and acupuncture group were significantly reduced after 6 weeks of treatment(P＜0.05),and the protein expression of MMP2 in acupuncture group was significantly reduced after 6 weeks of treatment(P＜0.05).4)Compared with Western medicine group,the protein expressions of Ang Ⅱ and ROCK1 in acupuncture group were significantly reduced after 6 weeks of treatment(P＜0.05). [Conclusion] Acupuncture at Renying Point can improve blood pressure and vascular injury in spontaneously hypertensive rats by down-regulating RhoA/ROCK1 pathway. |
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