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| Study on the mechanism of Astragalus polysaccharide alleviating muscle atrophy in diabetic sarcopenia rats |
| Hits 483 Download times 429 Received:August 05, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.01.20 |
| Key Words
Astragalus polysaccharide;diabetes;sarcopenia;animal experiment;PI3K-AKT-FOXO1 signaling pathway |
| Author Name | Affiliation | E-mail | | KONG Yihan | NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Institute of Endocrinology, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin 300134, China | | | LIU Xinbang | NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Institute of Endocrinology, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin 300134, China | | | CHANG Bai | NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Institute of Endocrinology, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin 300134, China | | | WANG Juan | NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Tianjin Institute of Endocrinology, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin 300134, China | wangjuanan6018@126.com |
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| Abstract
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| [Objective] To explore the therapeutic effect of Astragalus polysaccharide(APS) on muscle atrophy in diabetic sarcopenia rats by acting on PI3K-AKT-FOXO1 signaling pathway and related inflammatory factors. [Methods] The diabetic animal model was constructed by one-time intraperitoneal injection of streptozotocin,and was randomly divided into model group,APS group and metformin group. After the experiment,HE staining and Western Blot were used to determine P-AKT/AKT and FOXO1 protein content in gastnemius muscle,and fluorescence quantitative qPCR was used to detect IL-6,TP53,HSF1,MMP-9 and serum biochemical index TG.[Results] Compared with model group,the muscle cells of gastrocnemius muscle in APS group were significantly improved,the cell volume was enlarged,the space was narrowed,and the inflammatory exudation was significantly reduced. In addition,the levels of PAKT/AKT and FOXO1 protein were significantly increased in the APS group. The contents of inflammatory factor IL-6,TP53,heat shock transcription factor 1 and matrix metalloproteinase 9 decreased significantly. In terms of blood lipid,triglyceride decreased more significantly in the metformin group. [Conclusion] APS may reduce the levels of IL-6 and TP53 inflammatory factors through PI3K-AKTFOXO1 signaling pathway,affect the activities of heat shock transcription factor 1 and matrix metalloprotein-9,and thus improve the muscle atrophy in diabetic sarcosis rats. |
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