|
| A study of Danshen-Chuanxiong-Honghua on comorbidity of depression-heart injury in rats after traumatic brain injury |
| Hits 360 Download times 246 Received:November 04, 2023 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2024.02.12 |
| Key Words
traumatic brain injury;c-Fos;Ghrelin;BDNF;comorbidity of depression |
| Author Name | Affiliation | E-mail | | GAO Zhao | Nanjing University of Chinese Medicine, Nanjing 210023, China | | | SUN Bo | Nanjing University of Chinese Medicine, Nanjing 210023, China | | | CAI Yawen | Nanjing University of Chinese Medicine, Nanjing 210023, China | | | HUANG Xi | Nanjing University of Chinese Medicine, Nanjing 210023, China | 290606@njucm.edu.cn |
|
| Abstract
|
| [Objective] To explore the therapeutic effect and comorbidity mechanism of Danshen-Chuanxiong-Honghua and its main absorption component Ferulic acid on depression heart injury in traumatic brain injury（TBI） model rats. [Methods] SD rats were used to build traumatic brain injury model. After awakening，the model was treated with the medicine，and samples were taken after 24 h. Rats were divided into Sham group，Vehicle group，FA（2 mg/kg） group and DCH（10 g/kg） group. Using Golgi-Cox staining to measure the dendritic spine density and length in hippocampus neurons. Using ELISA to detect the c-Fos，Ghrelin in hippocampus，heart and serum，cardiac endothelial nitric oxide synthase（eNOS），Serum serotonin 5-HT，inflammatory factors interleukin IL-6，tumor necrosis factor TNF-α，Interleukin IL-β. Using Western blot to detect brain-derived neurotrophic factor BDNF expression in hippocampus and heart，cardiac injury index creatine kinase isoenzyme CK-MB and Myocardial apoptosis index Caspase3 expression in heart. [Results] After TBI，the length of hippocampal neurons and the density of dendritic spines decreased，inflammatory factors（IL-1β，IL-6 and TNF-α） in serum increased and 5-HT decreased. The level of eNOS in heart also decreased，the level of CK-MB and Caspase3 in heart increased. After treatment，the length of neurons in hippocampal increased，and the density of dendritic spines has a recovery trend. Inflammatory factors（IL-1β，IL-6 and TNF-α） in serum decreased，5-HT was increased. The level of eNOS in heart increased. CK-MB and Caspase3 in heart decreased. The expression levels of c-Fos and Ghrelin in the hippocampus，heart and serum have consistent expression characteristics. The expression level of the former increases after stress and decreases after treatment，the expression level of the latter decreased after stress and increased after treatment. BDNF appears to decrease after stress and increase after treatment in the heart and brain. [Conclusion] After TBI，rats exhibit depression-heart injury comorbidities. Both DCH and FA have antidepressant-cardioprotective effects and play a therapeutic role on the depression-heart injury comorbidities by regulating the shared pathway Ghrelin-BDNF/c-Fos. |
|
