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| Study on the mechanism of Qinghua Quyu Jianpi Decoction in treating ulcerative colitis based on network pharmacology and experimental verification |
| Hits 465 Download times 286 Received:December 04, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.02.16 |
| Key Words
Qinghua Quyu Jianpi Decoction;ulcerative colitis;network pharmacology;molecular docking;animal experiment |
| Author Name | Affiliation | E-mail | | QU Fanfan | Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China | | | SHEN Aihua | Beijing University of Chinese Medicine, Beijing 100029, China | | | ZHANG Shengsheng | Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China | zhangshengsheng@bjzhongyi.com | | LI Jilei | Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China | |
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| Abstract
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| [Objective] To investigate the potential pharmacological mechanism of Qinghua Quyu Jianpi Decoction in the treatment of ulcerative colitis from the perspective of network pharmacology and experimental validation. [Methods] Genes related to ulcerative colitis with Qinghua Quyu Jianpi Decoction were screened through the network database，further selected the shared genes，and performed GO function and KEGG enrichment analysis on the shared genes to determine the pharmacological effects of Qinghua Quyu Jianpi Decoction on ulcerative colitis. Meanwhile，a protein-protein interaction network and performed functional module analysis were established. The shared core genes were screened and the most stable binding molecules were selected for experimental validation by molecular docking. Finally，hematoxylin-eosin staining，immunohistochemical staining and Western blot assays were used to further confirm the molecular mechanism of the efficacy of the Qinghua Quyu Jianpi Decoction. [Results] The 86 active ingredients and 224 potential targets were screened from the formula，and 254 common targets were obtained by intersecting with the disease targets. These common targets were mainly involved in inflammatory response，DNA transcription，protein kinase activity，apoptosis，cell proliferation regulation and other processes，suggesting that Qinghua Quyu Jianpi Decoction could improve the inflammatory response by affecting these processes. Further，core gene analysis and molecular docking validation of the protein-protein interaction network were performed，and HIF1A，STAT3，MYC and MAPK3 were screened as possible main action targets of Qinghua Quyu Jianpi Decoction. Finally，MAPK3，the target with the most stable binding capacity predicted by molecular docking，was validated in animal experiments，and it was found that Qinghua Quyu Jianpi Decoction could significantly reduce the colonic inflammation response and promote MAPK3 protein expression in mice with ulcerative colitis. [Conclusion] The network pharmacology and molecular docking predicted the functional components and targets of Qinghua Quyu Jianpi Decoction for the treatment of ulcerative colitis，and animal experiments confirmed that the compound might activate MAPK signaling pathway and promote intestinal mucosal healing and play anti-inflammatory effect，which provides a new idea for the in-depth research of Qinghua Quyu Jianpi Decoction and other herbal compounds. |
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