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| Effect of polysaccharides in Tussilago farfara on the proliferation，migration and invasion of breast cancer cells induced by IL-6 by regulating miR-889-3p/TLR4 |
| Hits 321 Download times 266 Received:December 20, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.02.17 |
| Key Words
breast cancer;polysaccharides in Tussilago farfara;miR-889-3p;TLR4;interleukin 6;proliferation;migration |
| Author Name | Affiliation | E-mail | | ZHANG Huici | Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | | SHANG Qingxin | Teaching and Research Section of TCM Diagnostics, Shandong University of Traditional Chinese Medicine, Jinan 250355, China | shangqx0531@163.com |
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| Abstract
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| [Objective] To explore the effect and mechanism of polysaccharides in Tussilago farfara on breast cancer cell proliferation，migration and invasion induced by interleukin 6（IL-6）. [Methods] After IL-6 stimulation，MCF-7 cells were treated with different concentrations of polysaccharides in Tussilago farfara. The miR-NC and miR-889-3p mimics transfected MCF-7 cells were treated with IL-6；NC-inhibitor，miR-889-3p-inhibitors-transfected MCF-7 cells were treated with polysaccharides in Tussilago farfara and IL-6. Proliferation，migration，and invasion were assessed using MTT，plate clone formation，and TranswellTM experiments，miR-889-3p and TLR4 expression were detected using qRT-PCR. Western blot method was used to analysis of protein expression quantity of MMP-2，MMP-9 and TLR4. Dual luciferase reporter experiment verified the targeting between miR-889-3p and TLR4. [Results] Polysaccharides in Tussilago farfara could reduce IL-6-induced MCF-7 cell proliferation（P＜0.05），migration and invasion（P＜0.05），while miR-889-3p was increased（P＜0.05），in a dose-dependent manner. The miR-889-3p targeted TLR4 and regulated its expression. The miR-889-3p mimics reduced IL-6-induced MCF-7 cell proliferation，migration and invasion （P＜0.05）. The miR-889-3p-inhibitors restored the effects of polysaccharides in Tussilago farfara on MCF-7 cell proliferation，migration and invasion induced by IL-6. [Conclusion] Polysaccharides in Tussilago farfara inhibited breast cancer cell malignant behaviors induced by IL-6 through regulating miR-889-3p/TLR4 axis. |
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