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| Exploring the mechanism of Xiaoyan Decoction combined with apatinib in inhibiting the proliferation of gastric cancer MGC-803 cells based on the VEGF/PI3K/AKT pathway |
| Hits 262 Download times 136 Received:November 21, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.03.15 |
| Key Words
Xiaoyan Decoction;MGC-803 cell;gastric cancer;VEGF/PI3K/AKT |
| Author Name | Affiliation | E-mail | | MOU Ruiyu | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | LI Xiaojiang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | NIU Xiaofei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | LIAO Yang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | JIA Chunxin | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | KONG Fanming | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | GUO Shanqi | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | JIA Yingjie | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | jiayingjie1616@sina.com |
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| Abstract
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| [Objective] To investigate the antitumor mechanism of Xiaoyan Decoction on human gastric cancer MGC-803 cells. [Methods] The effect of Xiaoyan Decoction on the proliferation of MGC-803 cells was detected by CCK-8;The effect of Xiaoyan Decoction on apoptosis and cell cycle of MGC-803 cells was detected by flow cytometry;Western blot was used to detect the expression of VEGF/PI3K/Akt signal pathway related proteins after Xiaoyan Decoction,apatinib and their combination on MGC-803 cells. [Results] Xiaoyan Decoction inhibited the proliferation of MGC-803 cells in a dose-dependent manner(P<0.05),and its IC50 was 63.56 mg/mL; With the increase of Xiaoyan Decoction concentration,the total apoptosis rate of MGC-803 cells increased gradually (P<0.05);With the increase of Xiaoyan Decoction concentration,the percentage of cells in G0/G1 phase of MGC-803 cells decreased gradually(P<0.05),the percentage of cells in S phase increased gradually (P <0.05),and the percentage of cells in G2/M phase changed little (P>0.05); Compared with the blank control group,the expressions of p-PI3K,p-AKT,Bcl-2,VEGFA and VEGFR-2 proteins in Xiaoyan Decoction group,apatinib group and their combination group were significantly decreased (P<0.05),and the expressions of Cleaved Caspase-9 and Bax proteins were significantly increased(P<0.05). Compared with the single drug group,the combination group had a stronger regulatory effect on related proteins. [Conclusion] Xiaoyan Decoction can effectively inhibit the proliferation,induce apoptosis and S-phase block of human gastric cancer MGC-803 cells. The anti gastric cancer mechanism is related to its regulation of VEGF/PI3K/AKT signal pathway. |
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