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| Study on the mechanism of Shenqi Bushen Huoxue Decoction in improving delayed femoral fracture healing in rabbits |
| Hits 184 Download times 133 Received:September 20, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.03.16 |
| Key Words
delayed union of bone;Shenqi Bushen Huoxue Decoction;BMP-2;TGF-β1;VEGF |
| Author Name | Affiliation | E-mail | | FU Xuejing | Department of Orthopedics and Traumatology, Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China | | | ZHU Lin | Department of Orthopedics and Traumatology, Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China | | | ZHU Mingxi | Department of Orthopedics and Traumatology, Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China | Zmx19660115@163.com | | HAN Chongtao | Department of Orthopedics, Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450003, China | |
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| Abstract
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| [Objective] To explore the effect of Shenqi Bushen Huoxue Decoction on delayed femoral union in rabbits. [Methods] A delayed femoral union model was established by surgically removing 10 mm of cortical bone from the left middle femur of New Zealand white rabbits. The model animals were divided into model group,Shenqi Bushen Huoxue Decoction high-dose group (10 mL/kg) and Shenqi Bushen Huoxue Decoction low-dose group(5 mL/kg),with 10 animals in each group. Another 10 healthy animals were selected as control group. Shenqi Bushen Huoxue Decoction was intragastric for 4 weeks,intragastric administration was given once a day. The control group and model group were given normal saline with the same method. Serum samples and bone tissue samples of callus were collected from the sacrificed animals after drug administration. The relative expression levels of BMP-2,BMP-7,TGF-β1 and VEGF in serum were detected by RT-qPCR. The bone tissue healing was observed by hematoxylin-eosin staining. The expression levels of BMP-2, BMP-7,TGF-β1 and VEGF were detected by immunohistochemistry. [Results] Pathological results showed that the bone tissue in the model group healed slowly and the injured area was obvious,while in the Shenqi Bushen Huoxue Decoction high-dose group,there were obvious callus and bone tissue formation. Compared with control group,the expression levels of BMP-2,BMP-7,TGF-β1 and VEGF in serum and tissues of model group were decreased(P<0.01),compared with model group,the expression levels of BMP-2,BMP-7,TGF-β1 and VEGF in serum and tissues of Shenqi Bushen Huoxue Decoction at high dose were increased(P<0.05). [Conclusion] Shenqi Bushen Huoxue Decoction can promote the healing of rabbits femur,and its mechanism may be to promote the expression of BMP-2,BMP-7 and TGF-β1,and induce the formation of blood vessels in the bone defect area. |
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