|
| Effect of Shexiang Baoxin Pill on regulating PINK1/Parkin signaling pathway in diabetic retinopathy rats |
| Hits 697 Download times 360 Received:October 09, 2023 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2024.03.17 |
| Key Words
Shexiang Baoxin Pill;PINK1/Parkin signaling pathway;diabetic retinopathy;mitochondrial autophagy |
| Author Name | Affiliation | | YU Ling | Department of Endocrinology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | YANG Yang | Department of Endocrinology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | DUAN Chenghui | Department of Endocrinology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | DONG Ruihong | Department of Endocrinology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | SANG Yanhong | Department of Endocrinology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China |
|
| Abstract
|
| [Objective] To investigate the effect of Shexiang Baoxin Pill (SBP) on mitochondrial autophagy and PTEN-induced putative kinase 1(PINK1)/cytoplasmic E3 ubiquitin-ligase enzyme(Parkin) signaling pathway in diabetic retinopathy(DR) rats. [Methods] DR rats were separated into normal group(control group),model group(DR group),low dose Shexiang Baoxin Pill group(SBP-L group),high dose Shexiang Baoxin Pill group(SBP-H group),and high dose Shexiang Baoxin Pill+rapamycin group(SBP-H+RAP group). The levels of fasting blood sugar (FPG),total cholesterol (TC),triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) of blood lipids were detected in rats of each group;HE staining was applied to observe diabetic retinopathy of rats in each group;transmission electron microscopy was applied to observe the mitochondrial morphology of rat cells in each group;immunohistochemistry was applied to detect the expression of mitochondrial autophagy related proteins SQSTM1/P62,LC3-Ⅱ;Western blot was applied to detect the expression of PINK1 and Parkin proteins of rats in each group. [Results] Compared with the control group,the retinal structure of the DR group was severely damaged,with disordered cell arrangement,obvious edema,widened cell gaps,obvious mitochondrial damage and swelling,and abnormal morphology,the expression levels of FPG,TC,TG,LDL-C,LC3-Ⅱ,PINK1,and Parkin were obviously increased,the expression level of SQSTM1/P62 was obviously reduced(P<0.05);compared with the DR group,the structure of retinal cells in the SBP-L and SBP-H groups improved,the cell arrangement gradually became neat,the cell edema decreased,the morphology of cells and mitochondria tended to be normal,and mitochondrial swelling decreased,the expression levels of FPG,TC,TG,LDL-C,LC3-Ⅱ,PINK1, and Parkin were obviously reduced,the expression level of SQSTM1/P62 was obviously increased (P<0.05);compared with the SBP-H group,the structural damage of retinal cells in SBP-H+RAP group worsened,with disordered cell arrangement,obvious edema,increased mitochondrial swelling,and abnormal morphology,the expression levels of FPG,TC,TG,LDL-C,LC3-Ⅱ,PINK1,and Parkin were obviously increased,the expression level of SQSTM1/P62 was obviously reduced (P<0.05). [Conclusion] Shexiang Baoxin Pill can inhibit mitochondrial autophagy in diabetic retinopathy rats by inhibiting PINK1/Parkin signaling pathway. |
|
|
|
|
|
|
|
