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| Impacts of aucubin on viability and epithelial mesenchymal transformation of glioblastoma cells by regulating RhoA/ROCK signaling pathway |
| Hits 144 Download times 111 Received:September 21, 2023 |
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| DOI
10.11656/j.issn.1672-1519.2024.03.19 |
| Key Words
glioblastoma;cell viability;epithelial mesenchymal transformation;aucubin;RhoA/ROCK signaling pathway |
| Author Name | Affiliation | E-mail | | LI Juan | Department of Pharmaceutical, Suining Central Hospital, Suining 629000, China | | | SHI Haiping | Department of First Ward of Nerve Center, Suining Central Hospital, Suining 629000, China | | | LI Weimin | Department of First Ward of Nerve Center, Suining Central Hospital, Suining 629000, China | zeel57@163.com |
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| Abstract
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| [Objective] To investigate the impacts of aucubin(AU) on the viability and epithelial-mesenchymal transition(EMT) of glioblastoma (GBM) cells,and to explore its mechanism of action. [Methods] U87 were grouped into control group,low concentration group,medium concentration group,high concentration group,Y-27632 group,and high concentration+Y-27632 group. Cell counting kit-8 (CCK-8) method was applied to detect cell viability;flow cytometry was applied to detect cell apoptosis;Transwell cell experiment was applied to detect cell migration and invasion;Western blot was applied to detect the expression of matrix metalloproteinase (MMP) 2, MMP9,Vimentin,E-cadherin,N-cadherin,RAS homologous gene family member A (RhoA),Rho-related coiled protein kinase(ROCK) 1,ROCK2. The nude mouse model of GBM was constructed,and was grouped into nude mice control group,AU group,Y-27632 group, and AU +Y-27632 group;the mass and volume of tumors were measured,immunohistochemical method was applied to detect the expression of RhoA,ROCK1,and ROCK2 proteins in transplanted tumor tissue. [Results] The viability of GBM cells gradually decreased with the increase of AU concentration(P<0.05);U87 was selected as the follow-up experimental cells,and 10,25,50 μmol/L was selected as the follow-up experimental concentration of AU. Compared with the control group,the cell viability,migration and invasion cell counts,MMP2,MMP9,N-cadherin,Vimentin,RhoA,ROCK1,and ROCK2 expression in the low,medium,and high concentration AU groups and Y-27632 group were obviously decreased,the apoptosis rate and E-cadherin expression were obviously increased(P<0.05); the changes in high concentration AU and Y-27632 group were more obvious (P<0.05). Both AU and Y-27632 were able to inhibit the mass and volume of transplanted tumors,and reduce the expression of RhoA/ROCK signaling pathway proteins(P<0.05). [Conclusion] AU can inhibit the viability,migration,invasion and EMT of GBM cells and promote cell apoptosis,which may be related to the inhibition of RhoA/ROCK signaling pathway. |
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