|
| Mechanism of crocin treatment on improving diabetic nephropathy by inhibiting ferroptosis |
| Hits 231 Download times 108 Received:January 11, 2014 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2024.04.19 |
| Key Words
crocin;diabetic nephropathy;lipid peroxidation;ferroptosis |
| Author Name | Affiliation | E-mail | | CHEN Xiaoting | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | | | PAN Baochao | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | | | WANG Yuansong | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | | | SU Jinhao | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | | | CHEN Wei | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | | | JI Xiaohui | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | | | LYU Shuquan | Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province, Cangzhou 061000, China | czlvshuquan@163.com |
|
| Abstract
|
| [Objective] To investigate the effect of crocin（CRO） on diabetic nephropathy（DN） and explore its mechanism from the perspective of lipid peroxidation and ferroptosis. [Methods] Sixty C57BL/6J mice were adaptively fed for 1 week and then HFD for 8 weeks. After STZ modeling，the mice were randomly divided into control group，model group，ferroptosis inhibitor group（Fe-1，10 mg/kg，gavage），low dose CRO group（CROL，5 mg/kg，gavage），middle dose CRO group（CROM，10 mg/kg，gavage），high dose CRO group（CROH，20 mg/kg，gavage）. After 8 weeks of treatment，24 h urine samples，blood samples and kidneys were collected for analysis and determination. The levels of 24 h-UPQ，Cr and BUN in serum，MDA，ROS and 4-HNE in renal tissue were measured by kits to evaluate renal function and lipid peroxidation. HE，Masson and TUNEL staining were used to observe the pathological changes and cell death in the same part of the kidney. Level of Fe in renal was measured，and the expressions of STEAP3，TF，FTH1，FTL，and GPX4 were detected by RT-qPCR and Western blot to evaluate the effect of CRO on ferroptosis. [Results] Compared with the model group，the levels of Cr，BUN，24 h-UPQ decreased different degrees，and the pathological damage of renal tissue has different degrees of improvement，and the levels of MDA，ROS，4-HNE were significantly reduced，and TUNEL fluorescence positive reaction in renal tissue were significantly weakened，and the level of Fe in renal tissue decreased in different degrees after CRO treatment. The expression of STEAP3 and TF was down-regulated，while the expression of FTH1，FTL and GPX4 was up-regulated. [Conclusion] CRO can protect the kidney of DN mice by inhibiting lipid peroxidation and reducing ferroptosis. |
|
|
|
|
|
|
|
