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| Qinggan Huoxue Recipe regulates the cell pyroptosis mediated by Caspase-4/Caspase-3/GSDME to improve alcoholic liver injury |
| Hits 140 Download times 92 Received:January 11, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.06.17 |
| Key Words
Qinggan Huoxue Recipe;alcoholic liver disease;pyroptosis;Caspase-4/Caspase-3/GSDME pathway;traditional Chinese medicine compound |
| Author Name | Affiliation | E-mail | | WU Yan | Graduate School of Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China
Central Hospital of Minhang District, Shanghai 201199, China | | | SHENG Wei | Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China | | | WANG Lishun | Central Hospital of Minhang District, Shanghai 201199, China | | | ZHANG Li | Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China | | | JI Kang | Central Hospital of Minhang District, Shanghai 201199, China | | | CHEN Huijia | Central Hospital of Minhang District, Shanghai 201199, China | | | CHEN Junming | Graduate School of Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China
Central Hospital of Minhang District, Shanghai 201199, China | chjm2008@126.com |
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| Abstract
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| [Objective] To investigate the mechanism of Qinggan Huoxue Recipe（Qinggan Huoxue Recipe，QGHXR） in the prevention and treatment of alcoholic liver disease（ALD） by regulating Caspase-4/Caspase-3/GSDME pyroptosis pathway. [Methods] Eighteen C57BL/6 mice were randomly divided into control group，model group and QGHXR group to replicate the alcoholic liver disease model. Serum liver function and lipid levels were detected. Hematoxylin-eosin（HE） staining and oil red O staining were used to observe the pathological changes and lipid deposition in the liver. The expression of Caspase-4/Caspase-3/GSDME pathway related genes and proteins were detected by q-RT PCR，Western Blot and immunofluorescence staining. [Results] Compared with the control group，the liver TG level in model group was significantly increased（P<0.01），the serum ALT and AST levels were increased（P<0.05），and the serum IL-1β，IL-6 and TNF-α levels were increased（P<0.05）. Liver tissue showed pathological changes and lipid deposition. The mRNA expression levels of IL-1β，IL-6，TNF-α and NLRP3 in liver tissue were increased. Compared with model group，the liver TG level in QGHXR group was significantly decreased（P<0.01），and the serum ALT，AST，TBIL and TBA levels were decreased（P<0.05）. Serum TG，IL-6 and TNF-α levels were decreased（P<0.05） and serum HDL levels were increased（P<0.01）. Serum lipid，liver pathological changes and lipid deposition were relieved. The levels of GsdmeN，Caspase-4，Caspase-3 and C-Caspase-3 genes and proteins were decreased（P<0.05）. [Conclusion] QGHXR alleviates liver damage and lipid deposition in ALD mice by modulating the Caspase-4/Caspase-3/GSDME-mediated pyroptosis pathway. |
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