|
| Effect of Nrf 2/HO-1 signaling regulated by salvia miltiorrhiza polyphenols on oxidative stress in rats with membranous nephropathy |
| Hits 182 Download times 119 Received:January 22, 2024 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2024.07.20 |
| Key Words
miltiorrhiza polyphenols;membranous nephropathy;oxidative stress;Nrf 2/HO-1 signaling pathway |
| Author Name | Affiliation | E-mail | | PAN Huarong | Hebei University of Chinese Medicine, Shijiazhuang 050011, China
Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, China | | | GAO Shengning | Hebei University of Chinese Medicine, Shijiazhuang 050011, China
Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, China | | | SHEN Chenhui | Hebei University of Chinese Medicine, Shijiazhuang 050011, China
Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, China | | | TAN Miao | Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, China | | | TANG Miao | Second Hospital of Hebei Medical University, Shijiazhuang 050000, China | | | WANG Zhaoli | Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, China | | | TAN Jinchuan | Hebei University of Chinese Medicine, Shijiazhuang 050011, China
Hebei Hospital of Traditional Chinese Medicine, Shijiazhuang 050011, China | 1955981973@qq.com |
|
| Abstract
|
| [Objective] To investigate the effect of miltiorrhiza polyphenols on oxidative stress in membranous nephropathy（MN） rats based on the serum nuclear transcription factor 2/hemoglobin oxygenase-1（Nrf 2/HO-1） signaling pathway. [Methods] The 80 SD male rats were randomly selected as normal control group，and the remaining 60 rats were treated with tail vein injection of cated bovine serum albumin（C-BSA） to construct the MN model. MN rats were randomly divided into model group，benenapril hydrochloride（10 mg/kg），and polyphenols were divided into low，medium and high dose groups（16.7，33.3 and 66.7 mg/kg）. Drug gavage for 4 weeks，1 time/d，and equal volume of saline gavage in normal and model groups. Ururine protein quantification at 24 h（24 h UTP）. After the administration of the drug，blood urea nitrogen（BUN），serum creatinine（Scr），triglyceride（TG），total cholesterol（TC），total protein（TP），albumin（ALB） levels were measured；the renal histopathology was observed by silver overdate-hexidamine（PASM）；the deposition of immunoglobulin G（IgG） and complement C3 was detected by immunofluorescence；the expression of superoxide dismutase（SOD） and malondialdehyde（MDA） was detected by enzyme-linked immunosorbent assay（ELISA）；the expression of Nrf 2 and HO-1 was observed by protein immunoblotting（Western Blot）. [Results] Compared with the normal group，the glomeruli in the model group showed increased size，basement membrane thickening，the formation of “nail process”，diffuse deposition of complement C3 and IgG along the capillary haptics，UTP，serum TG and TC levels at 24 h significantly increased（P<0.01），and TP and ALB levels significantly decreased（P<0.01）. There was no significant difference between BUN and SCr；SOD expression was significantly decreased（P<0.01）；MDA expression was significantly increased（P<0.01）；Nrf 2，HO-1 mRNA and protein expression were significantly decreased（P<0.01）. Compared with the model group，24 h UTP，serum TG，TC were significantly decreased（P<0.05 or P<0.01），TP，ALB（P<0.05）；renal pathological damage was significantly improved；SOD（P<0.01），MDA（P<0.01）；Nrf 2，HO-1 mRNA and protein（P<0.05 or P<0.01）. [Conclusion] Salvia miltiorrhiza polyphenols may regulate the Nrf 2/HO-1 signaling pathway，and thus protect the kidney and delay the disease progression. |
|
|
|
|
|
|
|
