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| Study on the effect of polygonatum polysaccharides on the TGF-β/Smad signal pathway in placental tissue of pregnancy induced hypertension rats |
| Hits 179 Download times 161 Received:January 31, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.07.21 |
| Key Words
pregnancy induced hypertension;placental function;polygonatum polysaccharide;TGF-β/Smad signal pathway |
| Author Name | Affiliation | E-mail | | ZHOU Fangfang | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | LIU Yu | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | MA Shuangling | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | LI Xiaoqian | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | ZHANG Xinning | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | LI Cimei | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | BI Jingjing | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | | | LI Guoyun | Department of Obstetrics, Xinxiang Central Hospital, Xinxiang 453099, China | 2284499658@qq.com |
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| Abstract
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| [Objective] To investigate the impacts and mechanism of polygonatum polysaccharide on placental function in pregnancy induced hypertension model rats. [Methods] Pregnant female rats were randomly grouped into control group，model group，polygonatum polysaccharide（low，medium and high） dose groups（100，200，400 mg/kg） and magnesium sulfate group（100 mg/kg），with 10 rats in each group. In addition to the control group，rats in other groups were subcutaneously injected with L-arginine methyl ester to build the hypertensive model of pregnancy. Blood pressure，24-hour proteinuria，fetal rat body mass and placenta weight were measured；hematoxylin-eosin（HE） was applied to detect the histopathology of placenta；the contents of interleukin（IL）-6，IL-10 and tumor necrosis factor-α（TNF-α） in placenta were detected by enzyme linked immunosorbent assay（ELISA）；Western Blot was applied to detect the expression of tight junction protein-1（ZO-1），blocking protein（OCLN），vascular endothelial growth factor（VEGF），transforming growth factor-β1（TGF-β1），p-Smad3 and Smad3 proteins in placenta. [Results] Compared with the control group，the blood pressure，24-hour urine protein，IL-6，TNF-α contents and TGF-β1，p-Smad3 protein expression in the model group were significantly higher；the body weight，placenta weight，IL-10 content and the expression of ZO-1，OCLN and VEGF protein of fetal rats were significantly lower（P<0.05），and pathological damage occurred in placenta tissue. Compared with the model group，the blood pressure，24-hour urine protein，IL-6，TNF-α contents and TGF-β1，p-Smad3 protein expression of rats in administration groups were significantly lower，the body weight，placenta weight，IL-10 content and the expression of ZO-1，OCLN and VEGF protein of fetal rats were significantly higher（P<0.05），and the pathological damage of placenta was alleviated. [Conclusion] Polygonatum polysaccharide can inhibit the activation of TGF-β/Smad signal pathway in the placenta of pregnancy induced hypertension model rats and improve the placental dysfunction. |
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