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| Mechanism of nourishing yin and moistening intestine therapy for slow transit constipation based on PI3K/Akt pathway |
| Hits 164 Download times 103 Received:February 29, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.08.14 |
| Key Words
Ziyin Runchang Recipe;slow transit constipation;5-HT;Cajal interstitial cells;PI3K/Akt |
| Author Name | Affiliation | E-mail | | GE Shingning | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FAN Xuejin | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHANG Hanyu | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | LI Ying | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHANG Yanjun | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Traditional Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | ZHUANG Pengwei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | ZHANG Mixia | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YIN Qingsheng | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | carrysable@163.com | | GUO Hong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | cacti1983@163.com |
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| Abstract
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| [Objective] To explore the effect and mechanism of nourishing yin and moistening intestine in the treatment of slow transit constipation（STC） mice based on PI3K/AKT pathway. [Methods] The STC mice model was established by intragastric administration of loperamide hydrochloride. The 50 male ICR mice were randomly divided into normal group，model group，Ziyin Runchang Recipe low-dose group（1.3 g/kg），Ziyin Runchang Recipe high-dose group（2.6 g/kg） and positive drug group（Mosapili，3 mg/kg），with 10 mice in each group. Mice in each group were administrated by intragastric administration according to the corresponding drug concentration；normal group and model group were given equal doses of physiological saline by gavage，once a day while modeling，and fecal water content，time of first black defecation and intestinal transmission rate of mice in each group were detected after 14 consecutive days. The content of 5-hydroxytryptamine（5-HT） in colon tissue of each group was detected by enzyme-linked immunosorbent assay（ELISA）. The mRNA levels of SCF and c-kit in colon tissues of mice were detected by RT-PCR. The protein expression levels of c-kit，PI3K，p-PI3K，Akt and p-Akt in colon tissues of mice were detected by Western blot. [Results] Compared with normal group，the first black defecation time of mice in model group was significantly extended（P<0.05），fecal water content and intestinal transmission rate were significantly decreased（P<0.05），and colon 5-HT content，c-kit and SCF mRNA levels were significantly decreased（P<0.05）. The expression levels of c-kit，P-PI3K /PI3K and P-Akt /Akt in colon were significantly decreased（P<0.05）. Compared with the model group，fecal water content of Ziyin Runchang Recipe high-dose group was significantly increased（P<0.05），while that of Ziyin Runchang Recipe low-dose group and positive drug group had an increasing trend. The time of first black defecation in Ziyin Runchang Recipe high-dose group and positive drug group was significantly shortened（P<0.05），and the time of first defecation in Ziyin Runchang Recipe low-dose group had a trend of shortening. The intestinal transmission rate of Ziyin Runchang Recipe in all dose groups and positive drug groups was significantly increased（P<0.05）. In colon tissues，the mRNA levels of c-kit and SCF in Ziyin Runchang Recipe high-dose group were significantly increased（P<0.05），and the positive drug group had an increasing trend. The content of 5-HT and the expression of c-kit，p-PI3K /PI3K and p-Akt /Akt protein in Ziyin Runchang Recipe high-dose group and positive drug group were significantly increased（P<0.05）. [Conclusion] Ziyin Runchang Recipe can significantly improve intestinal transmission rate in the treatment of constipation，and its mechanism is related to increasing the content of 5-HT and the up-regulation of PI3K/Akt expression level，and then increasing the number of interstitial cells of Cajal. |
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