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Study on the influence of discoloration Baiji slices on its quality based on the UPLC fingerprint and multi-component chemometrics
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DOI   10.11656/j.issn.1672-1519.2024.08.16
Key Words   Baiji;chromatic value;fingerprint;militarine;canonical correlation analysis
Author NameAffiliationE-mail
ZHANG Jia College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China  
YUAN Jinfeng College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China  
CHAI Chongchong College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China  
SONG Yiyan College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China  
YAN Shuyu College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China  
CHEN Yilin College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China  
LIU Na College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China 837118072@qq.com 
LI Fei College of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China lf668@sina.com 
Abstract
    [Objective] To investigate the effect of green color on the quality of Baiji slices,and clarify the correlation between the contents of main chemical components and chromatic values. [Methods] The CM-5 colorimeter was used to measure the chromatic value of Baiji slices,the content of aflatoxin was detected by the fluorescence method,and UPLC fingerprint was also established for multi-component content determination. The OPLS-DA chemometric method was used to explore the differential biomarkers that affect the quality of Baiji slices,and the typical correlation analysis was performed between the chromatic value and the content of the biomarkers. [Results] By the comparison the chromaticity values,polysaccharides,and the content of eight characteristic peaks of five white,normal and green Baiji slices batches,it was found that polysaccharides,militarine,gymnoside Ⅲ,and dactylorhin A could be used to distinguish the normal and green colored Baiji slices. Typical correlation analysis shows that as the Baiji slices turn green,the L*,a*,and E* values decrease,while the b* value increases,and the contents of militarine,dactylorhin A,glucose Ⅲ,and polysaccharides also increase. [Conclusion] When the Baiji slices became green,the content of four main components,including polysaccharides,militarine,and others,significantly increased,and there was a significant correlation with chromaticity values. However,the impact of color changes on its pharmacological effects deserves further research.

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