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| Effects of Qidi Tongbian Prescription on mice with slow transit constipation based on Wnt/β-catenin pathway |
| Hits 39 Download times 31 Received:March 04, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.09.17 |
| Key Words
slow transit constipation;Qidi Tongbian Prescription;Wnt/β-catenin signaling pathway |
| Author Name | Affiliation | E-mail | | WANG Jiali | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | LI Junxiang | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | SHI Lei | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | klmysl1205@126.com | | MAO Tangyou | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | CHEN Runhua | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | HAN Xiao | Exchange, Development&Service Center for Science&Technology Talents, Beijing 100864, China | | | ZHANG Xiaosi | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | | | HU Juncong | Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China | |
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| Abstract
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| [Objective] To study the specific regulatory effects of Qidi Tongbian Prescription on slow transit constipation （STC） mice based on Wnt/β-catenin signaling pathway. [Methods] The 30 BALB/c mice were randomly divided into 6 groups，five mice in each group. Except the blank group，the STC mouse model was established by intragastric administration of loperamide hydrochloride （10 mg/kg） for 14 days，followed by intragastric intervention of high，medium and low doses（18.906，9.453，4.727 g/kg） of Qidi Tongbian Prescription and positive drug lactulose（3 g/kg） for 14 days. Fecal moisture content，fecal quantity and small intestine advancement rate were measured. Colon histological changes were evaluated by HE staining. Gene and protein expression levels of Wnt-3a and β-catenin were detected by PCR and immunohistochemistry. And the expression level of Ano1 protein in Cajal interstitial cells （ICC） was detected by immunofluorescence staining. The expression levels of Occludin，5-hydroxytryptamine（5-HT） and aquaporin 3（AQP3） were detected by ELISA. [Results] Compared with blank group，the small intestinal propulsion rate，fecal quantity and water content in model group were significantly decreased（P<0.01）. The intervention of Qidi Tongbian Prescription could effectively improve the small intestinal propulsion rate，fecal quantity and water content（P<0.05），and improve the histological lesions. Compared with blank group，the expression of Wnt-3a，β-catenin mRNA（P<0.05） and protein（P<0.01） increased in model group，while the expression of Ano1 protein decreased（P<0.01），Occludin protein decreased（P<0.01）. The protein expressions of AQP3（P<0.01） were increased. The prognosis showed that Wnt-3a protein，β-catenin mRNA and protein expression decreased（P<0.05），Ano1 protein expression increased（P<0.01），Occludin protein expression increased（P<0.01）. The protein expressions of AQP3 were decreased（P<0.05）. [Conclusion] Qidi Tongbian Prescription may play a role in the treatment of STC by inhibiting the overactivation of Wnt/β-catenin signaling pathway. |
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