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| HPLC-UV analysis of ginkgolide A,B,C |
| Hits 129 Download times 38 Received:February 23, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.09.18 |
| Key Words
ginkgolides;HPLC;methodology validation |
| Author Name | Affiliation | E-mail | | HU Zheming | Modern Chinese Medicine Discovery and Preparation Technology Engineering Center of the Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | PI Ziyue | Modern Chinese Medicine Discovery and Preparation Technology Engineering Center of the Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JOHN OSILAMA THOMAS | Modern Chinese Medicine Discovery and Preparation Technology Engineering Center of the Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GUO Pan | Modern Chinese Medicine Discovery and Preparation Technology Engineering Center of the Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | guopan@tjutcm.edu.cn |
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| Abstract
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| [Objective] To establish and methodologically validate an HPLC-UV method for the determination of ginkgolides A，B，and C. The method was validated by the HPLC-UV method. [Methods] The investigation included detection wavelength，eight different mobile phase systems and their ratios，injection volume，flow rate and column temperature. The method validation examined the specificity， precision，accuracy，linear range，repeatability，system suitability，spiked recovery and stability. [Results] The separation was carried out on an Agilent TC-5 C18 column at a detection wavelength of 220 nm with a mobile phase of water-methanol（70∶30） at an injection volume of 20 μL，a column temperature of 30 ℃，and a flow rate of 1.0 mL/min. The linearity of the method was good，and the peaks of the chromatograms did not interfere with each other. [Conclusion] The method is accurate，effective，economical，easy to obtain and simple to operate，and can provide a reference for the determination of other ginkgolides. |
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