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| Contents of 5 components in Xingnaojing Injection were determined by UPLC-MS/MS method |
| Hits 683 Download times 210 Received:April 23, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.09.19 |
| Key Words
Xingnaojing Injection;UPLC-MS/MS;content determination |
| Author Name | Affiliation | E-mail | | WANG Mochu | Tangshan Central Hospital, Tangshan 063000, China | | | FU Najing | Tangshan Central Hospital, Tangshan 063000, China | | | SHI Yanli | Tangshan Central Hospital, Tangshan 063000, China | | | CHEN Zhuo | Tangshan Central Hospital, Tangshan 063000, China | | | WANG Chuanqi | Tangshan Central Hospital, Tangshan 063000, China | | | LI Shuang | Tangshan Central Hospital, Tangshan 063000, China | | | MENG Fanshuang | Tangshan Central Hospital, Tangshan 063000, China | | | YAO Mengmeng | Jitang College of North China University of Science and Technology Tangshan 063000, China | | | JIANG Hui | Tangshan Central Hospital, Tangshan 063000, China | jianghui910@163.com |
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| Abstract
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| [Objective] A new method for simultaneous determination of 5 index components containing curcuma dione，gemarone，L-camphor，curcumin and muscosterone in Xingnaojing Injection was established by ultra high liquid chromatography-tandem masss pectrometry（UPLC-MS/MS）. [Methods] The chromatographic column used in this research was ACQUITY HSS? T3 C18 column（2.1 mm×100 mm，1.8 μm）. The mobile phase consisted of 0.1% formic acid water and 2 mmol ammonium acetate（A），methanol and 0.1% formicacid（B） with gradient elution（0 to 0.5 min，95% A；0.5 to 1.0 min，95% to 5% A；1.0 to 2.7 min，5% A；2.7 to 3.4 min，5%→95% A；3.4 to 4.0 min，95% A）. The injection volume was 5 μL and the flow rate was 0.4 mL/min. The column temperature was 40 ℃. Electrospray ion source was adopted for positive ion monitoring，and multi-reaction monitoring mode（MRM） was used for quantitative analysis.[Results] Within 4 min，the five signature components of Xingnaojing Injection，including curcuma dione，jimarone，L-camphor，curcumin and muscatone，were completely separated. The peak area showed a good linear relationship with the concentration，and the correlation coefficient（r） was greater than 0.998 0. The accuracy，repeatability and stability of the method were evaluated，and satisfactory results were obtained. The average recoveries were 98.2% to 104.2%. The contents of curcuma dione，jimarone，L-camphor，curcumin and muscatone in 6 batches of samples were 19.4 to 20.4，1.6 to 1.8，29.0 to 31.0，1.3 to 1.4，11.6 to 12.4 μg/mL，respectively. [Conclusion] The method has the advantages of high sensitivity，accuracy and reliability，and can realize the rapid detection of multiple components in Xingnaojing Injection，which provides a reference for the quality control research of this preparation. |
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