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| Mechanism study of resveratrol regulating LncRNA UCA1/Wnt/β-catenin signaling pathway inhibition of non small cell lung cancer |
| Hits 61 Download times 26 Received:April 07, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.09.20 |
| Key Words
resveratrol;LncRNA UCA1;lung cancer;Wnt/β-catenin pathway |
| Author Name | Affiliation | E-mail | | WANG Xiaodong | Oncology Department, Zibo Fourth People's Hospital, Zibo 255000, China | | | SHAO Junhuan | Oncology Department, Zibo Fourth People's Hospital, Zibo 255000, China | | | ZHANG Jiankun | Oncology Department, Zibo Fourth People's Hospital, Zibo 255000, China | 158256360@qq.com | | CUI Haiyan | Pathology Department, Zibo Fourth People's Hospital Zibo 255000, China | | | GENG Chao | Clinical Laboratory, Zibo Fourth People's Hospital, Zibo 255000, China | | | DENG Nan | Radiology Department, Zibo Fourth People's Hospital, Zibo 255000, China | | | TIAN Xia | Oncology Department, Zibo Fourth People's Hospital, Zibo 255000, China | |
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| Abstract
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| [Objective] Exploring how resveratrol regulates the expression of LncRNA-UCA1 in non-small cell lung cancer cells，thereby affecting the Wnt/β-catenin signaling pathway，ultimately inhibiting tumor cell proliferation，invasion，and promoting apoptosis，among other biological functions. [Methods] Firstly，investigate the effect of resveratrol on non-small cell lung cancer cell lines（A549 and Hcc827），and detect cell viability using CCK-8 assay kit；Transwell chamber and scratch experiments were conducted to verify the migration and invasion of tumor cells；detection of tumor cell apoptosis by flow cytometry；Western Blot was used to detect the expression of related pathway proteins （Wnt/β-catenin）. Subsequently，microarray technology was applied to screen for LncRNAs with abnormal expression in non-small cell lung cancer cells and normal human lung epithelial cells. A549 cells were co cultured with LncRNA-UCA1 overexpression and resveratrol to detect their effects on tumor cell proliferation，migration，in vasion，and apoptosis，as well as changes inWnt/β-catenin pathway related proteins. [Results] Resveratrol can inhibit the proliferation and invasion of non-small cell lung cancer cell lines（A549 and Hcc827） in a dose-dependent manner，promote their apoptosis，and downregulate the expression of β-catenin，CyclinD1，and c-myc proteins in the Wnt/β-catenin signaling pathway；meanwhile，LncRNA-UCA1 is highly expressed in non-small cell lung cancer cell lines，while resveratrol can significantly inhibit its expression；in addition，overexpression of LncRNA-UCA1 can promote the proliferation，migration，and invasion of non-small lung cancer cell lines（A549 and Hcc827） and inhibit cell apoptosis by regulating the Wnt/β-catenin signaling pathway. The addition of resveratrol can significantly inhibit these effects. [Conclusion] LncRNA-UCA1 is highly expressed in non-small cell lung cancer cell lines，and resveratrol can regulate the Wnt/β-catenin signaling pathway by downregulating its expression level to inhibit the proliferation，migration，and invasion of non-small cell lung cancer cells，and promote their apoptosis. |
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