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| Yiqi Huazhuo Decoction alleviates oxidative stress through PI3K/Akt pathway to treat insulin resistance in T2DM rats |
| Hits 48 Download times 34 Received:May 14, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.10.17 |
| Key Words
Yiqi Huazhuo Decoction;ROS;MDA;SOD;hepatic insulin resistance |
| Author Name | Affiliation | E-mail | | LI Yan | The Third School of Clinical Medicine, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China | | | WENG Siying | Ningbo Hospital of Traditional Chinese Medicine, Zhejiang University of Traditional Chinese Medicine, Ningbo 315010, China | alicewsy@163.com |
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| Abstract
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| [Objective] This study aimed to investigate the effect and mechanism of Yiqi Huazhuo decoction(YD) in treating insulin resistance(IR) in rats with type 2 diabetes mellitus(T2DM). [Methods] The low-dose YD group[15 mg/(kg·d)] and the high-dose YD group[30 mg/(kg·d)] were established as two intervention groups in this study. Blood glucose,serum insulin,and blood lipid levels of rats in the high-dose YD group were measured,and the homeostasis model assessment-insulin resistance(HOMA-IR) index was calculated. superoxide dismutase(SOD) and malondialdehyde(MDA) levels were determined using a biochemical method,while reactive oxygen species(ROS) levels were measured using an immunofluorescence method. Western blot analysis was conducted to assess the activity of phosphatidylinositol 3-hydroxy kinase(PI3K) and protein kinase B(Akt),aiming to observe the effects of YD intervention on PI3K-p85,Akt protein activity,downstream oxidative stress factors,and liver tissue injury. [Results] YD significantly reduced blood glucose levels,HOMA-IR index,and blood lipid levels in T2DM-IR rats. High-dose YD increased SOD level by 138.3% compared to the model group；it also decreased MDA and ROS levels by 43.4% and 45.2%,respectively. Additionally,high-dose YD reduced liver histomorphological score by 46.6% in the model group. Furthermore,there was a significant increase in relative expression ratio of p-PI3K-p85Tyr458/PI3K-p85 and p-AktS473/Akt proteins in liver tissues；these ratios reached 316.6% and 248.8%,respectively for high-dose YD within the model group. [Conclusion] YD can reduce damage caused by oxidative stress to the insulin target organ-liver-and improve liver IR by regulating glucose and lipid metabolism. The mechanism through which YD regulates hepatic IR may involve activating PI3K/Akt pathway as well as regulating downstream proteins’ expression along with antioxidant enzymes. |
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