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| Comparison the safety and efficacy of Breviscapine Injection and Synthetic Scutellarin Injection based on active systemic anaphylaxis in Hartley guinea pigs and inflammatory response in cerebral ischemia-reperfusion rats |
| Hits 6 Download times 3 Received:July 10, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.11.12 |
| Key Words
Breviscapine Injection;Synthetic Scutellarin Injection;active systemic anaphylaxis;cerebral ischemia reperfusion;inflammation |
| Author Name | Affiliation | E-mail | | ZHANG Yibo | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese medicine Pharmacology, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WU Shihao | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese medicine Pharmacology, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Feifan | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese medicine Pharmacology, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Zhangzhen | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese medicine Pharmacology, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHU Jinqiang | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Chinese medicine Pharmacology, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | zhujinqiang1860@163.com |
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| Abstract
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| [Objective] To compare the safety and anti-inflammatory efficacy of Breviscapine Injection(BI) and Synthetic Scutellarin Injection(SSI). [Methods] Active Systemic Anaphylaxis test in Hartley guinea pigs. Hartley guinea pigs were randomly divided into negative control group(sodium chloride injection),positive control group(bovine serum albumin),BI low dose(5 mg/kg) group,BI high dose(20 mg/kg) group,SSI low dose(5 mg/kg) group and SSI high dose(20 mg/kg) group,and were sensitized once every other day for 3 times,and stimulated on the 12th day after the last sensitization. And the symptoms of allergic reaction were observed. Cerebral ischemia-reperfusion inflammation test in rats. The middle cerebral artery embolization(MCAO) model of SD rats was prepared and they were divided into sham operation group,model group,Nimodipine group(1 mg/kg),BI-L(2.1 mg/kg),BL-H(4.2 mg/kg),SSI-L(2.1 mg/kg),and SSI-H(4.2 mg/kg) dose groups. The serum levels of tumor necrosis factor-α(TNF-α),intercellular adhesion molecule-1(ICAM-1),interleukin-6(IL-6),interleukin-1β(IL-1β) and interleukin-10(IL-10) were detected after 1,3,7 and 14 days of continuous administration. [Results] Active Systemic Anaphylaxis,in BI(5 mg/kg) group,1 was weakly positive and 5 were positive. In BI(20 mg/kg) group,3 were positive and 3 were strongly positive. SSI(5 mg/kg) was positive in 5 animals and strongly positive in 1 animal. In SSI(20 mg/kg) group,4 were positive,1 was weakly positive and 1 was negative. Cerebral ischemia-reperfusion inflammation test,compared with model group,after 1 day of administration,the volume of cerebral infarction in BI-L group was significantly decreased(P<0.01),and after 14 days of administration,the volume of cerebral infarction in Nimodipine,BI-L and SSI-H groups was significantly decreased(P<0.05 or P<0.01). After 1 day of administration,TNF-α content was significantly decreased in all other administration groups except the SSI-H group(P<0.05 or P<0.01). The contents of IL-6 and IL-1β were significantly decreased in all treatment groups except SSI-L group(P<0.05 or P<0.01). After 3 days of administration,IL-10 content was significantly increased in all groups except SSI-L group(P<0.05 or P<0.01). After 14 days of administration,the contents of TNF-α,ICAM-1 and IL-1β in administration groups were significantly decreased(P<0.05 or P<0.01). IL-6 content was significantly decreased in other administration groups except BI-L group(P<0.05 or P<0.01). IL-10 content was significantly increased in other administration groups except BI-L group(P<0.05 or P<0.01). [Conclusion] Lower incidence of allergic reactions and better safety in the high dose group of SSI. The effect of low-dose breviscapine group was faster,while the effect of high-dose synthetic scutellarin group was longer. |
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