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| Study on the effect and mechanism of Shengyang Yiwei Decoction in treating idiopathic membranous nephropathy based on the immune balance of Th17/Treg cells |
| Hits 5 Download times 4 Received:July 30, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.11.14 |
| Key Words
Shengyang Yiwei Decoction;membranous nephropathy;podocyte;immune balance;Th17/Treg |
| Author Name | Affiliation | E-mail | | WANG Shuaijie | Graduate School, Hebei University of Chinese Medicine, Shijiazhuang 050091, China | | | ZHANG Xueqin | Hebei University of Chinese Medicine, Shijiazhuang 050091, China | | | WU Yuan | Graduate School, Hebei University of Chinese Medicine, Shijiazhuang 050091, China | | | AN Hengtong | Graduate School, Hebei University of Chinese Medicine, Shijiazhuang 050091, China | | | MA Yun | Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang 050010, China | | | CHEN Zhiqiang | Hebei University of Chinese Medicine, Shijiazhuang 050091, China
Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang 050010, China | chenzhqiang2011@163.com |
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| Abstract
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| [Objective] Exploring the protective effect and mechanism of Shengyang Yiwei Decoction on membranous nephropathy(MN) rat model. [Methods] Forty male SD rats were randomly divided into control group with 10 rats and the modeling group with 30 rats. Rats were iv sheep anti-rat FX1A serum by tail to establish Heymann nephritis model. The rats with successful modeling were randomly divided into model group,Shengyang Yiwei Decoction group and Benazepril hydrochloride group,with 10 rats in each group,and the corresponding drugs were given by gavage. After 6 weeks of intervention,24 h-UTP,TP,Alb,TC,TG,Scr and BUN were detected,while renal tissue was stained with HE,Masson and PASM to observe the pathological changes of renal tissue,and Western blot method was used to detect the expression of IL-17A,IL-10,α-actinin-4,and Podocalyxin(Podxl) in renal tissue. Meanwhile,rat spleen was taken to detect the quantity of Th17 and Treg cells. [Results] Compared with the C group,the 24 h-UTP,TC,TG levels of rats in the M group were significantly higher,and TP and Alb levels were significantly lower(P<0.01),whereas the differences in Scr and BUN levels were not statistically significant(P>0.05). Meanwhile,renal pathological changes were obvious in the M group,and α-actinin-4 and Podxl expression were significantly decreased(P<0.01,P<0.05). Additionally,IL-17A significantly increased and IL-10 decreased(P<0.01). And Th17% and Th17/Treg were significantly higher and Treg% was significantly lower(P<0.01). Compared with the M group,the levels of 24h-UTP,TC,TG,and TP were significantly reduced(P<0.01,P<0.05) and the level of Alb was significantly increased(P<0.05) in each treatment group. Simultaneously the pathological changes of kidney were improved. Among them,the expression of α-actinin-4 and Podxl was significantly increased in the Z group(P<0.05,P<0.01),while IL-17A,Th17% and Th17/Treg were significantly reduced,and IL-10 and Treg% were increased(P<0.01,P<0.05). [Conclusion] Shengyang Yiwei Decoction can reduce proteinuria,attenuate renal pathological injury and increase the expression of α-actinin-4 and Podxl in podocytes expressing,which has obvious renal protective effects in rats with membranous nephropathy. In addition,the mechanism of these protective effects may be related to the regulation of Th17/Treg immune balance. |
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