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| Research on antidepressant effect and mechanism of magnet processed regulating intestinal microbiota-neurotransmitter/inflammatory factors |
| Hits 8 Download times 5 Received:August 03, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.11.16 |
| Key Words
magnet;processing;depression;intestinal flora;neurotransmitters;inflammation |
| Author Name | Affiliation | E-mail | | SONG Xuejiao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIN Wenhan | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHUANG Pengwei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | YIN Qingsheng | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | carrysable@163.com | | CHAI Shiwei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | chaishiwei791109@sina.com |
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| Abstract
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| [Objective] To clarify the effect of calcined magnet on its antidepressant effect,and to explore its mechanism based on intestinal flora-neurotransmitter/inflammatory factors. [Methods] Chronic unpredicted mild stress(CUMS) was used to induce anxiety and depression-like behavior in rats. The rats were divided into blank group,model group,raw magnet group,calcined magnet group and positive control group. The rats in the raw magnet group,calcined magnet group and positive control group were given 3.15 g/kg,3.15 g/kg and 0.63 mg/kg raw magnet,calcined magnet and estazolam by intragastric administration every day. The rats in the blank group and the model group were given equal volume of distilled water for 3 weeks. Sucrose preference test,open field test and elevated plus maze test were used to evaluate the anxiety and depression behavior of rats. The contents of monoamine neurotransmitters dopamine(DA),5-hydroxytryptamine(5-HT),norepinephrine(NE) and 5-hydroxyindole acetic acid(5-HIAA) in cortex were detected by HPLC-ECD. The levels of inflammatory factors interleukin-18(interleukin-18,IL-18)、interleukin-6(interleukin-6,IL-6) and interleukin-1β(interleukin-1β,IL-1β) mRNA in hippocampus were detected by qPCR. The changes of intestinal microbial structure and composition were analyzed by 16S rRNA sequencing. [Results] Compared with the normal group,the sucrose preference rate,the number of rearing in the open field,the number and time of entering the open arm of the elevated plus maze in the model group were significantly decreased(P<0.05),the contents of cortical neurotransmitters(5-HT,NE and 5-HIAA) were significantly decreased(P<0.05),the mRNA levels of hippocampal pro-inflammatory factors(IL-18,IL-6 and IL-1β) were significantly increased(P<0.05),and the structure and composition of intestinal flora were disordered. Compared with the model group,the sucrose preference rate and the number and time of entering the open arm of the elevated plus maze were significantly increased in the raw and calcined magnet groups(P<0.05). The number of upright times in the open field of rats in the calcined magnet group increased(P<0.05). The content of neurotransmitters in the cerebral cortex of rats in the calcined magnet group increased,and the abundance of Lactobacillus, Limosilactobacillus and Bacteroides in the intestinal bacteria increased significantly (P<0.05). The mRNA levels of pro-inflammatory factors(IL-6 and IL-1β) in the hippocampus of rats in the raw magnet group decreased significantly(P<0.05). The abundance of Christensenellaceae_R-7_group,NK4A214_group and Roseburia in intestinal bacteria increased significantly. [Conclusion] Both raw and calcined magnet can improve the anxiety and depression-like behavior of CUMS rats. Calcined magnet is related to the increase of brain neurotransmitter content by regulating intestinal Lactobacillus and Bacteroides,while raw magnet is related to the regulation of intestinal Roche bacteria and other pro-inflammatory factors. |
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