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| Mechanism of Fufang Shangtong Capsule in the treatment of femoral shaft fracture based on animal experiment and network pharmacology |
| Hits 5 Download times 1 Received:July 28, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.11.17 |
| Key Words
femoral shaft fracture;Fufang Shangtong Capsule;network pharmacology |
| Author Name | Affiliation | E-mail | | YANG Jialin | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Chao | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | LIU Aifeng | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | draifeng@163.com | | HE Qi | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | NIU Puyu | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | |
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| Abstract
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| [Objective] To explore the potential mechanism of Fufang Shangtong Capsule in the treatment of femoral shaft fracture based on animal experiment and network pharmacology. [Methods] The rat model of femoral shaft fracture was established,and the Micro-CT results,biomechanical analysis results,serum IL-6 levels,serum TNF-α levels and the number of VEGF mRNA positive cells were observed after taking Fufang Shangtong Capsule. The active ingredients and targets of drugs of Fufang Shangtong Capsule were screened by TCMSP database. The related targets of Femoral Shaft Fracture were searched by Genecards and OMIM databases,and their intersection with the targets of drugs were selected as potential targets of Fufang Shangtong Capsule for the treatment of Femoral Shaft Fracture. The visual "drug-ingredient-target" network was constructed by Cytoscape. The protein-protein interaction(PPI) network was obtained by the String database. The bioinformatics analysis were carried out for these potential targets by the Metascape database. [Results] Animal experiment indicated that the rats in the Fufang Shangtong Capsule treatment group recovered well under Micro-CT scanning,and the biomechanical test results showed that the maximum load of the femoral shaft in the treatment group was higher than that in the control group. Fufang Shangtong Capsule can reduce the expression of IL-6 and TNF-α,and increase the expression of VEGF in rats with femoral shaft fracture. The 209 active ingredients and 60 potential targets for the treatment of femoral shaft fracture were obtained from the Fufang Shangtong Capsule in the research results of network pharmacology. The top 5 target genes with degree in the PPI network were ALB,AKT1,IL-6,TP53 and VEGFA. Gene Ontology(GO) enrichment analysis enriched a total of 452 GO entries,and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis yielded 23 pathways associated with the treatment of femoral stem fractures. [Conclusion] This study preliminarily elucidates the mechanism of Fufang Shangtong Capsule in the treatment of femoral shaft fracture with multi-components,multi-targets and multi-pathways to provide a basis for its further development and utilization. |
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