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| Study on the effect of Shenjing Fuyuan Decoction on androgen receptor expression and activity of hippocampal neurons in poststroke depression rats |
| Hits 8 Download times 1 Received:June 05, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.12.15 |
| Key Words
Shenjing Fuyuan Decoction;post-stroke depression;androgen receptor;hippocampal neurons;rat |
| Author Name | Affiliation | E-mail | | WU Zicong | Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China | | | CAI Li | Department of Encephalopathy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China | cailiys@163.com | | ZHAO Yanqing | Department of Encephalopathy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China | | | LI Wentao | Department of Encephalopathy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China | |
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| Abstract
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| [Objective] This study aims to investigate the impact of Shenjing Fuyuan Decoction on androgen receptor（AR） expression and activity in rats with post-stroke depression（PSD），and to provide a basis for exploring its mechanism of action. [Methods] The PSD rat model was established by using classical wire bolus to the middle cerebral artery plus chronic unforeseen mild stress combined with the solitary rearing method. Subsequently，the rats were divided into a sham operation group，a PSD model group，a Shenjing Fuyuan group，and a fluoxetine group. A sugar preference test，open field test，and Morris water maze were used to observe the behavioral changes of rats. After 14 days of drug intervention，hippocampal tissue was collected from rats to obtain neuronal cells，and the expression of phosphorylated AR and non-phosphorylated AR（p-AR） in cells was detected using immunofluorescence and protein immunoblotting methods. [Results] On the 14th day of stress，the glucose consumption，and open field test horizontal and vertical scores of the model group，fluoxetine group，and Shenjing Fuyuan group were significantly reduced compared to before surgery，and the model was successfully established. After 14 days of drug intervention，the glucose consumption，horizontal and vertical scores of rats in the fluoxetine group and the Shenjing Fuyuan group significantly increased compared to the 14th day of stress，and were considerably higher than those in the model group. Compared with the model group，the two groups of rats had a shorter latency for navigation in the Morris water maze，a longer duration of spatial search in the target quadrant，and a significant increase in the number of crossings. The expression levels and protein expression levels of AR and p-AR in cells were significantly increased compared to the model group. [Conclusion] Shenjing Fuyuan Decoction improves depressive symptoms and enhances learning memory ability in PSD model rats，and its mechanism of action may be related to the promotion of AR activation. |
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