|
| To investigate the mechanism by which modified Jiawei Fangji Huangqi Decoction intervenes in the lncRNA NKILA-mediated RIF model of HK-2 cells to inhibit EMT |
| Hits 11 Download times 2 Received:October 18, 2024 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2024.12.16 |
| Key Words
Fangji Huangqi Decoction;lncRNA NKILA;RIF;JAK2/STAT |
| Author Name | Affiliation | E-mail | | HAN Yu | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin Institute of Traditional Chinese Medicine, Tianjin 300120, China | | | WANG Yaogaung | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | wangyaoguang1012@126.com |
|
| Abstract
|
| [Objective] To explore the intervention mechanism of the modified Fangji Huangqi Decoction on the epithelial-mesenchymal transformation in the HK-2 cell fibrosis model mediated by lncRNA NKILA. [Methods] Modified Fangji Huangqi Decoction was applied in an HK-2 cell RIF model built by lncRNA NKILA overexpression lentivirus transfection，with an AG490 control group. qPCR，Western Blot，and immunofluorescence were utilized to assess the alterations in lncRNA NKILA expression，EMT markers FN，Col1，Vim，α-SMA，and JAK2/STAT3 pathway indicators. [Results] EMT phenotypic index analysis indicated up-regulated mRNA and protein levels of FN，Col1，Vim，and α-SMA in the oe-NKILA group versus the oe-NC group，with a noTab.down-regulation in E-cad mRNA and protein levels. Compared to the oe-NKILA group，the oe-NKILA+AG490 and oe-NKILA+TCM-H groups exhibited significant down-regulation in E-cad mRNA and protein levels，with statistical significance（P<0.05）. The expression of lncRNA NKILA in each group was also examined. Compared to the oe-NC group，NKILA expression was significantly elevated in the oe-NKILA group. However，NKILA expression was significantly reduced in the oe-NKILA+AG490 group（P<0.01） and the oe-NKILA+TCM-H group（P<0.05）. The JAK2/STAT3 pathway indicator analysis revealed significant up-regulation in mRNA and protein，p-JAK2，and p-STAT3 protein expressions of JAK2 and STAT3 in the oe-NKILA group versus the oe-NC group，with significant down-regulation in the oe-NKILA+AG490 and oe-NKILA+TCM-H groups（P<0.05）. [Conclusion] The high-dose serum of the modified Fangji Huangqi Decoction can alleviate the EMT injury of HK-2 cells induced by lncRNA NKILA overexpression and inhibit the activation of the JAK2/STAT3 signaling pathway during the process of lncRNA NKILA-inducing EMT of HK-2 cells. |
|
