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| Study on the regulation of BMP2/RUNX2 signaling pathway by Cuscuizi Pills in the treatment of postmenopausal osteoporosis |
| Hits 11 Download times 3 Received:July 10, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2024.12.19 |
| Key Words
postmenopausal osteoporosis;cuscuta;hyssop;osteogenesis-related factors |
| Author Name | Affiliation | E-mail | | GUO Xiaotong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SONG Junli | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WEI Qiu | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Jie | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MAO Haoping | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | haoping_mao@126.com |
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| Abstract
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| [Objective] To investigate the effect and mechanism of Cuscuta pills（CP） on postmenopausal osteoporosis（PMOP）. [Methods] A mouse model of postmenopausal osteoporosis was established using bilateral ovarian extraction（OVX）. The mice were randomly divided into sham operation group（Sham），model group（OVX），estrogen 39 μg/kg group（Estrogen），Cuscuta 4 g/kg group（T 4 g/kg），hyssop 4 g/kg group（N 4 g/kg），and Cuscuta pill 8 g/kg group（CP 8 g/kg），which were administered continuously for 12 weeks， with 10 animals in each group. Dual-energy X-ray absorptiometry was used to detect mice’s bone mineral density（BMD）. YLS-16A small animal bone strength tester to measure tibial strength；viva CT40 to detect femoral bone microstructure；hematoxylin-eosin（HE） staining was used to observe the pathological morphology of bone tissue. Enzyme-linked immunosorbent assay（ELISA） was used to detect the contents of alkaline phosphatase（ALP），bone-specific alkaline phosphatase（BALP），and osteoproteectin（OPG） in plasma. Western Bolt was used to detect the expression of OPG，RUNX2 and BMP2 proteins in the bone marrow of castrated mice. [Results] Compared with the Sham group，the BMD and tibial bone strength of the femur in the OVX group were significantly reduced（P<0.01），a large number of trabecular bones were missing and the bone structure was sparse，the levels of ALP，BALP and OPG in plasma and the protein expression of OPG，RUNX2 and BMP2 in bone marrow were significantly reduced（P<0.01），while CP 8g/kg group significantly increased the femoral BMD and tibial bone strength of OVX mice（P<0.01），significantly increased the levels of osteogenesis-related factors ALP，BALP and OPG in plasma，and increased the protein expressions of RUNX2，OPG and BMP2 in bone tissue（P<0.01），and its effect is significantly higher than that of Cuscuta chinensis and hyssop treated alone（P<0.05）. [Conclusion] Cuscuta pills significantly inhibited bone loss caused by castration. They up-regulated the expression of osteogenesis-related factors BALP，RUNX2，and OPG，suggesting that its anti-postmenopausal osteoporotic effect may be played by promoting bone formation through the BMP2/RUNX2 signaling pathway. |
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