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| Effect of rutin on TGF-β1 induced fibrosis of cardiac fibroblasts |
| Hits 668 Download times 253 Received:September 20, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.01.14 |
| Key Words
rutin;cardiac fibrosis;cardiac fibroblasts;TGF-β1/Smad signaling pathway |
| Author Name | Affiliation | E-mail | | ZHANG Guannan | School of Life Sciences, Ningxia University, Yinchuan 750021, China | | | NIU Pilian | School of Life Sciences, Ningxia University, Yinchuan 750021, China | | | JING Ruixin | School of Life Sciences, Ningxia University, Yinchuan 750021, China | | | SHI Xinwei | Shanxi Institute of Botany, Xi'an 710061, China | | | BAI Mingsheng | School of Life Sciences, Ningxia University, Yinchuan 750021, China | baimingsheng@163.com |
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| Abstract
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| [Objective] To explore the effect of rutin on transforming growth factor-β1（TGF-β1）-induced fibrosis of cardiac fibroblasts（CFs） and the TGF-β1/Smad signaling pathway. [Methods] The CFs cell model induced by TGF-β1 was constructed，and the groups were set as follows：control group（complete medium+cells），model group（containing 10 ng/mL TGF-β1+cells），positive control group（10 ng/mL TGF-β1+10 μmol/L captopril and cells） and administration group（10 ng/mL TGF-β1+200 μg/mL rutin and cells）. The expression of α-smooth muscle actin（α-SMA），Vimentin（Vim），Fibronectin（FN），Collagen I，Collagen Ⅲ and TGF-β1/Smad pathway-related proteins were detected by Western blot. The gene expression levels of Acta 2，Vim，FN，Col Ⅰ1a1，matrix metalloproteinase 2（MMP-2） and matrix metalloproteinase 9（MMP-9） were detected by quantitative real-time polymerase chain reaction（qPCR）. Immunofluorescence was used to detect the expression and localization of the key protein α-SMA to explore the effect of rutin on TGF-β1-induced CFs fibrosis. [Results] After CFs were induced by TGF-β1，the expression of Vim，FN，Collage I，Collage Ⅲ，TGF-β1/Smad signaling pathway-related proteins was successfully activated. After intervention with 200 μg/mL rutin，the activation of Vim，FN，CollageⅠ，Collage Ⅲ，TGF-β1/Smad signaling pathway-related proteins and mRNA was inhibited，thereby alleviating the occurrence of myocardial fibrosis. The results of immunofluorescence assay showed that compared with the control group，the green fluorescence intensity of α-SMA in the TGF-β1 model group was significantly enhanced. Compared with the model group，the green fluorescence intensity of α-SMA was significantly weakened in the 200 μg/mL rutin group. [Conclusion] The results of this study showed that 200 μg/mL rutin alleviated myocardial fibrosis by inhibiting the expression of Vim，FN，Collage I，Collage Ⅲ，α-SMA and TGF-β1/Smad signaling pathway-related proteins and mRNA induced by TGF-β1. |
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