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| The mechanism of total glucosides of paeony on chronic immune thrombocytopenia |
| Hits 35 Download times 18 Received:August 20, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.01.16 |
| Key Words
total glucosides of paeony;immune thrombocytopenia;mice;immunity;platelet |
| Author Name | Affiliation | E-mail | | ZHENG Jing | Department of Rheumatology and Immunology, Inner Mongolia Ordos Central Hospital, Ordos 017000, China | | | REN Shuiming | Department of Rheumatology and Immunology, Inner Mongolia Ordos Central Hospital, Ordos 017000, China | nzg534014@163.com | | GAO Qian | Department of Rheumatology and Immunology, Inner Mongolia Ordos Central Hospital, Ordos 017000, China | | | GAO Jing | Department of Rheumatology and Immunology, Inner Mongolia Ordos Central Hospital, Ordos 017000, China | |
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| Abstract
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| [Objective] To investigate the mechanism of total glucosides of paeony on chronic immune thrombocytopenia. [Methods] Sixty BALB/C mice with SPF grade were divided into control group（n=10），model group（n=9），total glucosides of peony（TGP） low dose group（n=10），TGP medium dose group（n=9），TGP high dose group，and Western medicine group（n=10） according to random number method. Immune thrombocytopenia（ITP） animal models were established in both the Western medicine group（n=10） and the Western medicine group（n=10） except the control group. After modeling，24 h TGP low，medium，and high dose groups were given a TGP solution of 100 mg/kg，200 g/kg，and 400 mg/kg，while the Western medicine group was given 5 mg/kg prednisone acetate. The other groups were given the same volume of normal saline，and the platelet count was detected by automatic biochemical analyzer before modeling，24 h after modeling，1 week of intervention，2 weeks of intervention，3 weeks of intervention，and 4 weeks of intervention. The percentages of helper T cells 17（Th17） and regulatory T cells（Teg） were detected by flow cytometry. The levels of interleukin-10，IL-17，and transforming growth factor（TGF） -β1 were detected by an enzyme-linked immunosorbent kit. Hematoxylin-eosin（HE） staining was used to observe the pathological morphology of liver and spleen tissue and to calculate the organ index. [Results] At 24 hours after modeling and 1 week of intervention，compared to the control group，the platelet counts in all other groups of mice decreased（P<0.05），but there was no significant difference among the groups（P>0.05）. At 2，3，and 4 weeks of intervention，compared to the control group，the platelet counts in the model group decreased（P<0.05），while the platelet counts in the TGP low，medium，high dose groups，and Western medicine group increased compared to the model group（P<0.05）. The platelet counts gradually increased in the TGP low，medium，and high dose groups，and Western medicine group，with significant differences observed among the groups（P<0.05）. Compared to the control group，the percentage of Treg in the peripheral blood of mice in the model group decreased，while the percentage of Th17，liver index，and spleen index increased（P<0.05）. Compared to the model group，the TGP low，medium，and high dose groups，and Western medicine group showed an increase in the percentage of Treg，and a decrease in the percentage of Th17，liver index，and spleen index（P<0.05）. Compared to the control group，the levels of IL-10 and TGF-β1 in the peripheral blood of mice in the model group decreased，while IL-17 increased（P<0.05）. Compared to the model group，the TGP low，medium，and high dose groups，and Western medicine group showed an increase in IL-10 and TGF-β1 levels，and a decrease in IL-17 levels（P<0.05）. IL-17 showed differences among the TGP low，medium，and high dose groups（P<0.05），while there was no difference in TGF-β1 levels among the TGP low，medium，high dose groups and western medicine group（P>0.05）. There was no difference in IL-17 levels between the TGP high dose group and the Western medicine group（P>0.05）. [Conclusion] Total glucoside of paeony can improve immune function，increase platelet count，and reduce liver and spleen injury in mice with chronic immune thrombocytopenia. |
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