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| Study on the mechanism of action of traditional Chinese medicine Xingnao Zhitan Capsule in the treatment of blood stasis due to qi deficiency type of stroke based on network pharmacology and experimental validation |
| Hits 30 Download times 17 Received:August 14, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.01.17 |
| Key Words
Xingnao Zhitan Capsule;qi deficiency;blood stasis;stroke;network pharmacology;molecular docking |
| Author Name | Affiliation | E-mail | | TAN Peiyi | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Chinese Medicine Acupuncture and Moxibustion Clinical Medical Research Center, Tianjin 300381, China | | | TIAN Guang | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Chinese Medicine Acupuncture and Moxibustion Clinical Medical Research Center, Tianjin 300381, China | | | OUYANG Huizi | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Chinese Medicine Acupuncture and Moxibustion Clinical Medical Research Center, Tianjin 300381, China | | | CHAI Shiwei | The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Chinese Medicine Acupuncture and Moxibustion Clinical Medical Research Center, Tianjin 300381, China | chaishiwei791109@sina.com |
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| Abstract
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| [Objective] The traditional Chinese medicine formula Xingnao Zhitan Capsule has been widely used in the treatment of various types of strokes with blood stasis due to qi deficiency，but its overall pharmacological mechanism of action has been relatively little studied. In this paper，we explored the overall pharmacological mechanism of Xingnao Zhitan Capsule using network pharmacology，molecular docking，and experimental validation. [Methods] It was searched in TCMSP and TCM-ID databases for active chemical components with oral bioavailability greater than 30% and drug-like properties greater than 0.18 and their corresponding targets of action for each flavor of traditional Chinese medicine in Xingnao Zhitan Capsule. It was searched in DrugBank，GeneCards，OMIM，TTD and other databases for targets corresponding to blood stasis due to qi deficiency type of stroke. A potential target network of Chinese medicine-active ingredient- blood stasis due to qi deficiency type of stroke was constructed using Cytoscape software for Xingnao Zhitan Capsule. A protein-protein interaction networks was constructed using STRING software. Target GO function enrichment analysis and KEGG pathway enrichment analysis were performed using the DAVID program. Software such as AutoDock Vina and Discovery Studio Visualizer were used to perform molecular docking and analyze patterns of action. The effects of the extract of Xingnao Zhitan Capsules on human neuroblastoma cells（SH-SY5Y） injury induced by oxygen-glucose deprivation/reoxygenation（OGD/R），such as cell survival rate，lactate dehydrogenase（LDH） release rate，and the regulation of some genes expression. [Results] Network pharmacological analysis showed that PTGS2，PTGS1，HSP90AA1，NCOA2，ADRB2、CHRM1 are the potential main targets for the efficacy of Xingnao Zhitan Capsules. Compounds such as quercetin，kaempferol，luteolin，and other compounds are the potential active ingredients in the pharmacological effect of Xingnao Zhitan Capsule. Molecular docking revealed potential binding possibilities of Luteolin to β2-adrenergic receptor（ADRB2），quercetin to cyclooxygenase-2（PTGS2），baicalein to M1 receptor（CHRM1），and cryptotanshinone to cyclooxygenase-1（PTGS1）. The extract of Xingnao Zhitan Capsules dose-dependently repaired OGD/R damage to SH-SY5Y cells，increased cell survival，decreased LDH release rate and down-regulated OGD/R-induced high expression of cellular PTGS2/PTGS1/HSP90AA1 mRNA and protein. [Conclusion] The results of network pharmacology research，molecular docking combined with experimental validation provide a new research direction for elucidating the material basis of the efficacy of Xingnao Zhitan Capsules and the overall pharmacological mechanism. |
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