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| Exploring the mechanism of Bazi Bushen Capsule in delaying age-related macular degeneration based on network pharmacology and in vitro experimental validation |
| Hits 556 Download times 292 Received:August 28, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.01.19 |
| Key Words
Bazi Bushen Capsule;age-related macular degeneration;autophagy;cellular aging;in vitro experiment;retinal epithelial cell;network pharmacology |
| Author Name | Affiliation | E-mail | | SHU Rongli | School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China | | | GONG Boyang | School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Zhaodong | School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China | | | TAN Yawen | School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHAO Xue | First Clinical Medical College, Xinjiang Medical University, Urumqi 830011, China | | | ZHANG Jiani | Basic Medicine School of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China | | | ZHOU Huifang | School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China | | | HOU Yunlong | Hebei Medical University, Shijiazhuang 050011, China | houyunlonghrb@hotmail.com | | BIAN Yuhong | School of Integrated Traditional Chinese and Western Medicine, Tianjin Universty of Traditional Chinese Medicine, Tianjin 301617, China | bianyuhong_2012@163.com |
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| Abstract
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| [Objective] To investigate the mechanism of Bazi Bushen Capsules（BZBS） in delaying age-related macular degeneration（AMD） based on network pharmacology and validate it in vitro. [Methods] Constructing a visualization relationship diagram of “traditional Chinese medicine-disease-key target” of BZBS in the treatment of AMD through network pharmacology；GO analysis and KEGG signaling pathway enrichment analysis were performed on the key targets. The freeze-dried powder of BZBS was used to intervene in ARPE-19 cells for in vitro experiments，and whether it could improve D-gal-induced ARPE-19 cellular senescence was examined by ROS staining，β-galactosidase staining（SA-β-gal），Western blot，the ELISA. And validate the signaling pathway results predicted by network pharmacology. [Results] The network pharmacology screening yielded a total of 293 main active ingredients of BZBS，including quercetin，farnesolone A，stigmasterol，and arachidonic acid；the 58 key targets of BZBS and AMD were predicted. in vitro experiments showed that BZBS could effectively increase the viability of senescent ARPE-19 cells，down-regulate the degree of reactive oxygen species，reduce the senescence-related secretion phenotype，and up regulate the expression of P62，and LC3，key proteins of autophagy signaling pathway（P<0.05）. [Conclusion] BZBS can delay the aging process of AMD，and its mechanism may be related to the regulation of autophagy signaling pathway. |
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