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| Anti-tumor effect of glycyrrhetinic acid modified shikonin liposomes combined with photothermal therapy on hepatoma carcinoma cells |
| Hits 34 Download times 7 Received:September 24, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.02.13 |
| Key Words
liposome;shikonin;liver cancer;photothermal therapy |
| Author Name | Affiliation | E-mail | | WANG Zhengyu | The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China | | | CHEN Qingshan | The First Clinical Chinese Medicine College of Hunan University of Chinese Medicine, Changsha 410007, China | s_mountain@126.com | | TAN Xinhua | The First Clinical Chinese Medicine College of Hunan University of Chinese Medicine, Changsha 410007, China | 115744971@qq.com |
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| Abstract
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| [Objective] To prepare a novel multifunctional liposome GA-Shikonin-IR783-lliposome（GSIL） loaded with Shikonin/IR783 and coated with Glycyrrhetinic acid，which can specifically target liver cancer cells. The physical and chemical properties and in vitro anti-tumor efficacy of the multifunctional liposome were evaluated in this study. [Methods] The GSILs were prepared using the thin film-rehydration method，and their particle size，surface potential，polymer dispersion index（PDI），electron microscope morphology，stability，encapsulation rate，and photothermal properties were measured. At the cellular level，the cellular uptake behavior of GSIL and SIL was investigated by confocal microscopy using human hepatoma cell HepG2. The synergistic effect of photothermal/anti-hepatocellular carcinoma in vitro was confirmed through cytotoxicity tests. [Results] AThe GSIL loaded with shikonin and IR783 were effectively formulated for targeted delivery. Under the electron microscope，a circular structure was observed，indicating good stability and photothermal properties with an encapsulation rate of （53.65±4.43）%，average particle size of（209.67±2.88） nm，average potential of （-40.83±0.68） mV，and PDI of 0.20 ±0.01. Compared to free shikonin，GSIL exhibits a higher cellular uptake by HepG2 cells and significantly enhances the inhibitory effect when combined with near-infrared laser irradiation on HepG2 cells. [Conclusion] A novel GSIL has been successfully synthesized，demonstrating excellent photothermal/anti-tumor synergistic effects. This offers a new perspective on the treatment of liver cancer through the combination of nano-Chinese medicine and photothermal ablation. |
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