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| Exploring the protective effect of Qianggan Ruanjian pill on liver fibrosis in rats based on TGF-β1/Smads signaling pathway |
| Hits 46 Download times 17 Received:October 20, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.02.15 |
| Key Words
Qianggan Ruanjian pill;TGF-β1/Smad pathway;liver fibrosis;liver |
| Author Name | Affiliation | E-mail | | PING Jiayi | Kaifeng Hospital of Traditional Chinese Medicine, Kaifeng 475002, China | | | LIU Jingsheng | Kaifeng Hospital of Traditional Chinese Medicine, Kaifeng 475002, China | jsl6969@126.com | | REN Zhiguang | Institute of Traditional Chinese Medicine, Henan University, Kaifeng 475004, China | |
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| Abstract
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| [Objective] To investigate the protective effect of Qianggan Ruanjian pill on the liver of rats with liver fibrosis（HF） based on the growth factor-β1（TGF-β1）/Smads signaling pathway. [Methods] Wistar rats were separated into a control group，HF group，Qianggan Ruanjian pill group，colchicine group，SRI-011381 group，and Qianggan Ruanjian pill+SRI-011381 group，with 12 rats in each group. Except for the control group，rats in all other groups were injected intraperitoneally with a 50% carbon tetrachloride（CCl4） olive oil solution of 1.5 mL/kg to construct HF models. After successful modeling，medication was administered once a day for 6 weeks. The activity of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in serum，and the contents of type Ⅳ collagen（CIV），type Ⅲ procollagen（PC-Ⅲ），laminin（LN），and hyaluronic acid（HA） were detected；hematoxylin-eosin（HE） and Masson staining were applied to detect pathological changes in liver tissue and liver fibrosis，respectively；immunohistochemistry was applied to detect the number of α-smooth muscle actin（α-SMA） positive cells in liver tissue；Western blot was applied to detect the expression of TGF-β1 and p-Smad2/3 proteins in liver tissue. [Results] ACompared with the control group，the HF group had severe liver tissue pathological damage and liver fibrosis，the activities of ALT and AST in serum，the contents of CIV，PC-Ⅲ，LN，HA，the number of α-SMA positive cells in liver tissue，and the expression of TGF-β1 and p-Smad2/3 proteins increased（P＜0.05）；compared with the HF group，the liver tissue pathological damage and liver fibrosis in the Qianggan Ruanjian pill group and the colchicine group were improved，the activities of ALT and AST in serum，the contents of CIV，PC-Ⅲ，LN，HA，the number of α-SMA positive cells in liver tissue，and the expression of TGF-β1 and p-Smad2/3 proteins decreased，the change trend of corresponding indicators in SRI-011381 group was opposite to the above（P＜0.05）；compared with the Qianggan Ruanjian pill group，the pathological damage and liver fibrosis of the liver tissue in the Qianggan Ruanjian pill+SRI-011381 group were exacerbated，the activities of ALT and AST in serum，the contents of CIV，PC-Ⅲ，LN，HA，the number of α-SMA positive cells in liver tissue，and the expression of TGF-β1 and p-Smad2/3 proteins increased（P＜0.05）. [Conclusions] Qianggan Ruanjian pill may improve HF in rats by inhibiting the TGF-β1/Smads signaling pathway. |
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