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| Exploring the mechanism of Qihuang Jiedu Huayu Yin in treating acute kidney injury in sepsis based on TLR4/MyD88/NF-κB signal pathway |
| Hits 717 Download times 185 Received:August 29, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.02.16 |
| Key Words
sepsis;acute kidney injury;Qihuang Jiedu Huayu Yin;TLR4/MyD88/NF-κB signal pathway |
| Author Name | Affiliation | E-mail | | SHI Yuexin | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China
Beijing University of Chinese Medicine, Beijing 100029, China | | | YAO Zhi | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China
Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Li | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China | | | ZHU Rui | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China
Beijing University of Chinese Medicine, Beijing 100029, China | | | YAN Jun | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China | dzmyyyj@126.com | | WU Caijun | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, China | |
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| Abstract
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| [Objective] To explore the mechanism of Qihuang Jiedu Huayu Yin in treating sepsis-induced acute kidney injury（AKI） based on TLR4/MyD88/NF-κB signal pathway. [Methods] SD rats were randomly divided into sham group，model group，and low，medium，and high dose groups of Qihuang Jiedu Huayu Yin，with 10 rats in each group. The sepsis-induced AKI rat model was constructed by cecal ligation and puncture. Each group was orally administered every 12 hours before modeling for 3 consecutive days，and 12 hours after modeling. Record the general status of each group of rats，and use an ELISA kit to detect serum creatinine（Scr），urea nitrogen（BUN），neutrophil gelatinase-associated lipid releasing hormone（NGAL），kidney injury molecule-1（KIM-1），and interleukin-1β（IL-1β）、IL-6，IL-10，tumor necrosis factor（TNF）-α，catalase（CAT），glutathione peroxidase（GSH-PX），malondialdehyde（MDA），and total superoxide dismutase（T-SOD）. Observation of pathological morphological changes in rat kidney tissue using hematoxylin-eosin（HE） staining. The expression levels TLR4，MyD88，NF-κB in rat kidney tissue protein were detected by Western blot. [Results] ACompared with the model group，the general state of rats in the low，medium，and high-dose groups of Qihuang Jiedu Huayu Yin was improved. The serum levels of Scr and BUN were significantly reduced in the high-dose group（P<0.05），while the levels of NGAL and KIM-1 were significantly reduced in the medium and high-dose groups（P<0.05 or P<0.01）. The pathological damage of renal tissue in low，medium，and high dose groups of Qihuang Jiedu Huayu Yin was significantly improved；the level of IL-1β，IL-6，TNF-α in Low，medium，and high-dose groups of Qihuang Jiedu Huayu Yin were decreased（P<0.01 or P<0.05），while the level of IL-10 was increased（P<0.01）. The levels of MDA in the low，medium，and high dose groups of Qihuang Jiedu Huayu Yin were significantly reduced（P<0.01），while the level of CAT was significantly increased（P<0.05 or P<0.01）. The levels of GSH-PX significantly increased in the medium and high dose groups（P<0.05）. The T-SOD level in the high-dose group significantly increased（P<0.05）. In low，medium，and high-dose groups of Qihuang Jiedu Huayu Yin，the expression level of TLR4，MyD88，and NF-κB were significantly reduced（P<0.01 or P<0.05）. [Conclusion] Qihuang Jiedu Huayu Yin can improve the general state of sepsis-induced AKI rats，improve renal dysfunction and pathological damage，reduce inflammation levels and oxidative stress response，and its mechanism of action may be related to the inhibition of TLR4/MyD88/NF-κB signal pathway. |
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