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| Effect of Sanhuang Yishen Capsule on oxidative stress in rats with diabetic nephropathy based on Nrf2/HO-1 signaling pathway |
| Hits 44 Download times 10 Received:October 07, 2024 |
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| DOI
10.11656/j.issn.1672-1519.2025.03.17 |
| Key Words
Sanhuang Yishen Capsule;diabetic kidney disease;oxidative stress;Nrf2/HO-1 signaling pathway |
| Author Name | Affiliation | E-mail | | FAN Lirong | Botou Hospital of Traditional Chinese Medicine, Botou 062150, China | | | LI Songlin | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | LI Hanzhou | College of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Hui | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | WU Rui | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | LIU Zhen | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | SU Xiuhai | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | LI Jianxiu | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | WANG Yanan | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | BIAN Yuhong | College of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LYU Shuquan | Cangzhou Hospital of Combined Traditional Chinese and Western Medicine, Cangzhou 061000, China | czlvshuquan@163.com |
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| Abstract
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| [Objective] To study the therapeutic effect of Sanhuang Yishen Capsule on diabetic nephropathy（DKD） model rats and its effect on Nrf2/HO-1 signaling pathway. [Methods] Forty male SD rats of SPF grade were selected. Eight were randomly chosen as normal control group，and the rest of the rats were injected with high-fat chow and streptozotocin（STZ） to establish the DKD model rats，which were randomly divided into the model group，the Sanhuang Yishen Capsule low（0.81g/kg） and high（1.62 g/kg） dose group. The positive group was given Irbesartan（30 mg/kg） by gavage，while the normal group and the model group were given equal volume of distilled water by gavage for four weeks. Firstly，the therapeutic effect of Sanhuang Yishen Capsule on DKD model rats was evaluated by examining the relevant biochemical indexes and renal histopathological changes in the serum and urine of rats in each group after modeling. Secondly，the levels of antioxidant-related indexes of superoxide dismutase（SOD），malondialdehyde（MDA），and glutathione peroxidase（GSH-PX） were detected in the renal tissues of the rats in each group，so as to clarify the therapeutic effect of Sanhuang Yishen Capsule on DKD model rats. Meanwhile the levels of interleukin（IL）-1β，IL-6 and tumor necrosis factor α（TNF-α） in the homogenates of renal tissues of rats in each group were detected by ELISA to clarify the effect of Sanhuang Yishen Capsule on the inflammatory response of renal tissues of rats in the DKD model. Finally，qPCR and Western blot were used to detect the levels of Nrf2 and HO-1 genes and proteins，which are key factors of Nrf2/HO-1 signaling pathway，in the renal tissues of rats in each group，so as to preliminarily investigate the mechanism of inhibition of oxidative stress by Sanhuang Yishen Capsule in the renal tissues of rats in the DKD model. [Results] Sanhuang Yishen Capsule could significantly reduce the levels of Cr and BUN in the serum of DKD model rats，reduce the 24 h urinary protein（UTP） level，and improve the focal degeneration of renal tubules，glomerular basal pathological changes such as membrane thickening simultaneously. In addition，Sanhuang Yishen Capsule can significantly increase the activities of SOD and GSH-PX in the kidney tissue of DKD model rats，reduce the level of MDA，and reduce the levels of IL-6，IL-1β and TNF-α in the serum of DKD rats. The results of qPCR and Western blot showed that Sanhuang Yishen Capsule could significantly up-regulate the gene and protein levels of Nrf2 and HO-1 in the kidney tissue of DKD model rats. [Conclusion] Sanhuang Yishen Capsule has a therapeutic effect on DKD model rats，and its mechanism may be related to up-regulating Nrf2/HO-1 signaling pathway in renal tissue and improving its oxidative stress. |
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