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| Mechanism research of salvianolic acids treating myocardial fibrosis |
| Hits 585 Download times 237 Received:January 13, 2025 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2025.05.13 |
| Key Words
myocardial fibrosis;salvianolic acids;collagen deposition;action mechanism |
| Author Name | Affiliation | E-mail | | MA Yaolei | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHOU Fengjie | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Xiao | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HUO Xiaowei | College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China | huoxiaoweiforever@163.com | | LEI Wei | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | l_wei8910@163.com |
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| Abstract
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| [Objective] Myocardial fibrosis can disrupt cardiac structure，leading to heart failure and adversely affecting the prognosis of patients with heart failure. Existing treatments have limited efficacy in improving myocardial fibrosis. Danshen，a traditional herb known for its blood-activating and stasis-eliminating properties，is widely used in the prevention and treatment of cardiovascular diseases. This study aims to investigate the inhibitory effects of salvianolic acid（a major active compound from Danshen） on myocardial fibrosis and its underlying mechanisms through in vitro cell experiments and network pharmacology analysis. [Methods] This study employed a combination of in vitro experiments and network pharmacology. In the in vitro experiments，salvianolic acid（25 to 100 μg/mL） was applied to rat cardiac fibroblasts undergoing phenotypic transformation induced by 10 ng/mL TGF-β1 and to H9c2 cells stimulated by TGF-β1/Ang Ⅱ. The pharmacological effects of salvianolic acid on myocardial fibrosis were explored using q-PCR and Western blot. In the network pharmacology analysis，the mass spectrometry results and myocardial fibrosis targets were integrated for comprehensive analysis. [Results] Under TGF-β1 stimulation，salvianolic acid dose-dependently reduced the expression of COLIII，COLI，and α-SMA，inhibiting the phenotypic transformation of cardiac fibroblasts. Additionally，salvianolic acid suppressed the overexpression of COLIA1，COL3A1，MMP2，and MMP9 in H9c2 cells induced by TGF-β1/Ang Ⅱ. UPLC-Q-TOF-MS identified 24 chemical components in salvianolic acid. Network pharmacology analysis revealed that the target genes related to myocardial fibrosis were mainly enriched in lipid and atherosclerosis processes，as well as the PI3K-AKT signaling pathway. [Conclusion] This study，through molecular biology experiments，UPLC-Q-TOF-MS，and network pharmacology analysis，clarified the effects of salvianolic acid in inhibiting the phenotypic transformation of cardiac fibroblasts and reducing the secretion of pro-fibrotic factors in H9c2 cells. It also elucidated the potential pharmacological substances and mechanisms of salvianolic acid，providing a scientific basis for its application in treating myocardial fibrosis. |
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