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| Mechanistic study of Jianpi Qinghua Formula in improving obesity by suppressing adipose tissue inflammation |
| Hits 596 Download times 270 Received:February 18, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.05.16 |
| Key Words
Jianpi Qinghua Formula;obesity;inflammation |
| Author Name | Affiliation | E-mail | | HUANG Hui | Department of Endocrinology, Shuguang Hospital, Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | XU Jiahui | Department of Traditional Chinese Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | LU Hao | Department of Endocrinology, Shuguang Hospital, Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | GONG Fan | Department of Endocrinology, Shuguang Hospital, Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | 372438536@qq.com |
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| Abstract
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| [Objective] To evaluate the applicability and efficacy of Jianpi Qinghua Formula（JPQH） in inhibiting adipose tissue inflammation and improving obesity and its associated metabolic disorders using diet-induced and genetic obesity models. [Methods] The gene expression omnibus（GEO） database was used to analyze the correlation between obesity，visceral adipose tissue inflammation，and the expression of inhibitor of nuclear factor kappa-B kinase ε（IKKε）. Diet-induced obesity（DIO） and leptin receptor-deficient diabetic（db/db） mice were divided into normal control，model，and JPQH groups. The effects of JPQH on body weight，adipose tissue mass，oral glucose tolerance test（OGTT），and intraperitoneal insulin tolerance test（IPITT） in mice were assessed. quantitative polymerase chain reaction（qPCR） was used to detect the transcription levels of tumor necrosis factor-alpha（TNF-α） and interleukin-6（IL-6） in visceral adipose tissue，and Western blot was employed to measure IKKε expression. [Results] Analysis of the GEO database indicates that obesity is closely associated with elevated expression of the inflammatory factor TNF-α and IKKε in visceral adipose tissue across both in vivo and in vitro models. In DIO mice，treatment with JPQH significantly reduced body weight（P＜0.01） and adipose tissue mass（P＜0.001） compared to the model group. Additionally，JPQH improved glucose metabolism，as evidenced by a significant reduction in the area under the curve（AUC） following OGTT and IPITT intervention experiments（P＜0.001）. The qPCR and Western blot results demonstrated that JPQH treatment significantly inhibited the expression of inflammatory factor TNF-α，IL-6，and IKKε in visceral adipose tissue（P＜0.01 or P＜0.05）. Similarly，in db/db mice，JPQH treatment significantly reduced body weight and adipose tissue mass compared to the model group（P＜0.05），improved AUC following the OGTT intervention（P＜0.05），markedly decreased TNF-α and IL-6 mRNA levels in visceral adipose tissue（P＜0.01 or P＜0.001），and inhibited IKKε expression（P＜0.05）. [Conclusion] JPQH reduces body weight and improves glucose metabolism in obese mice by inhibiting adipose tissue inflammation，demonstrating broad applicability in both diet-induced and genetically predisposed obesity. |
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