|
| Correlation between preoperative traditional Chinese medicine syndromes，clinicopathological features，and NLRP3 expression in papillary thyroid carcinoma |
| Hits 516 Download times 217 Received:January 14, 2025 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2025.06.04 |
| Key Words
traditional Chinese medicine syndromes;papillary thyroid carcinoma;NOD-like receptor pyrin domain-containing 3 inflammasome;clinicopathological characteristics |
| Author Name | Affiliation | E-mail | | HAN Shiyu | Diabetes Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | SHANG Wenbin | Endocrinology Department, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China | | | CHEN Qingguang | Diabetes Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | LU Hao | Diabetes Institute, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | luhao403@163.com |
|
| Abstract
|
| [Objective] To analyze the clinicopathological characteristics of patients with papillary thyroid carcinoma（PTC） before surgery and the distribution of traditional Chinese medicine syndromes. Furthermore，to examine the relationship between traditional Chinese medicine syndromes and expression of NOD-like receptor pyrin domain-containing 3（NLRP3） in PTC tissues. [Methods] A total of 190 patients with PTC who received surgical treatment in Affiliated Hospital of Nanjing University of Chinese Medicine from July 2022 to March 2023 were selected as the research subjects，and the preoperative diagnostic was divided into three types：stagnation of liver-qi，phlegm-stasis cementation，heat and blood stasis syndrome. The clinicopathological features of the patients（sex，age，tumor size，pathological type，condition of metastatic lymph nodes and invasion of surrounding tissues，and tumor number） were collected. Protein expression in PTC tissues was determined by immunohistochemistry. The distribution characteristics of traditional Chinese medicine syndromes and the correlation between syndrome types and NLRP3 expression were statistically analyzed. [Results] In the study，including 190 cases of PTC，81 cases（42.6%） had phlegm-stasis intertwining syndrome，61 cases（32.1%） had stagnation of liver-qi，and 48 cases（25.3%） had heat and blood stasis. The differences of age，gender，tumor number and invasion of surrounding tissues between all syndromes were not statistically significant（P>0.05）. The proportion of patients with stagnation of liver-qi syndrome in pathological type Ⅰ and the proportion of patients with heat and blood stasis syndrome in pathological type Ⅱ to Ⅳ were higher than those with phlegm-stasis intertwining syndrome syndrome（P<0.05）. The rate of metastatic lymph nodes was higher in patients with heat and blood stasis than other patients（P<0.05）. Compared with phlegm-stasis intertwining syndrome syndrome，the largest tumor size in patients with stagnation of liver-qi were more often <0.8 cm，and patients with heat and blood were more often≥0.8 cm（P<0.05）. The positive expression rate of NLRP3 in PTC patients was 64.2%，with higher positive rates and immunohistochemical score in PTC tissues of patients with heat and blood stasis（P<0.05）. The expression of NLRP3 showed statistically significant differences（P<0.05） in both liver-qi stagnation syndrome and phlegm-stasis intermingling syndrome，depending on the presence of surrounding tissue invasion and whether the maximum tumor diameter exceeded 0.8 cm. In heat and blood stasis syndrome，significant differences in NLRP3 expression were observed between patients with and without lymph node metastasis and surrounding tissue invasion（P<0.05）. [Conclusion] The stagnation of liver-qi and phlegm-stasis intertwining syndrome were the most TCM syndrome in PTC patients. The positive expression of NLRP3 contribute to discriminate the heat and blood stasis syndrome from the phlegm-stasis cementation and stagnation of liver-qi syndromes，and NLRP3 expression was associated with certain post-operative clinicopathological characteristics in three syndromes. |
|
