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| Investigating the effect of total paeony glycoside on airway inflammation in mice with neutrophilic asthma based on the AMPK/Sirt1 pathway |
| Hits 537 Download times 249 Received:January 25, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2025.06.16 |
| Key Words
total paeony glycoside;AMPK/Sirt1 pathway;neutrophils;asthma;airway inflammation |
| Author Name | Affiliation | E-mail | | WU Linlin | Department of Respiratory Medicine, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450053, China | | | SUN Xiaomin | Department of Respiratory Medicine, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450053, China | 13513892805@163.com | | YAN Xingyu | Department of Respiratory Medicine, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450053, China | | | HUANG Han | Department of Respiratory Medicine, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450053, China | |
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| Abstract
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| [Objective] To investigate the effect of total paeony glycoside（TPG） on airway inflammation in mice with neutrophilic asthma based on the adenosine monophosphate-activated protein kinase（AMPK）/silent information regulator 1（Sirt1） pathway. [Methods] Asthmatic mice were prepared by nasal instillation of ovalbumin sensitization solution. The mice were randomly grouped into sensitization group，TPG low-dose（TPG-low，150 mg/kg TPG） group，TPG high-dose（TPG-high，300 mg/kg TPG） group，dexamethasone（4 mg/kg） group，and TPG-high+inhibitor（15 mg/kg） group. Subsequently，nebulization challenge was performed. Mice in the control group were injected with normal saline into the nasal cavity and challenged with atomized normal saline. After 1 hour，airway resistance was analyzed. Bronchoalveolar lavage fluid（BALF） was collected，and cells were classified and counted. The levels of inflammatory cytokines interleukin（IL）-6，tumor necrosis factor-α（TNF-α），and IL-17 in BALF were analyzed. The lung tissue was isolated，and the pathology of lung tissue，changes in airway mucus，AMPK phosphorylation（p-AMPK）/AMPK，and Sirt1 protein expression were detected. [Results] Compared with the control group，the expiratory pause，number of inflammatory cells such as neutrophils，the levels of IL-6，IL-17，TNF-α，inflammatory score，and PAS staining score increased in the sensitization group，while the expression of p-AMPK/AMPK and Sirt1 decreased（P<0.05）. Compared with the sensitization group，the expiratory pause，number of inflammatory cells such as neutrophils，levels of IL-6，IL-17，TNF-α，inflammatory score，and PAS staining score decreased in the TPG-low group，TPG-high group，and dexamethasone group，while the expression of p-AMPK/AMPK and Sirt1 increased（P<0.05），and significant differences were observed among TPG groups（P<0.05）. Compared with the TPG-high group，the expiratory pause，number of inflammatory cells such as neutrophils，the levels of IL-6，IL-17，TNF-α，inflammatory score，and PAS staining score increased in the TPG-high+inhibitor group，while the expression of p-AMPK/AMPK and Sirt1 decreased（P<0.05）. [Conclusion] TPG alleviates airway inflammation in mice with neutrophilic asthma by upregulating the AMPK/Sirt1 pathway. |
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