|
| Based on the adiponectin signaling pathway of skeletal muscle discussing the effect of Shenqi compound on improving the abnormal glucose and lipid metabolism and skeletal muscle injury in aging diabetes rats by treating from the perspective of spleen |
| Hits 226 Download times 85 Received:August 31, 2025 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2026.01.15 |
| Key Words
Shenqi compound;diabetes;senility;skeletal muscle;adiponectin |
| Author Name | Affiliation | E-mail | | TIAN Yuan | Chengdu Sport University, Chengdu 641418, China
Chengdu Medical College, Chengdu 610500, China | | | CHAO Jun | Chengdu Medical College, Chengdu 610500, China | | | ZHU Haiyan | Chengdu Medical College, Chengdu 610500, China | | | ZHONG Wen | Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China | zhongwenzwcd@163.com |
|
| Abstract
|
| [Objective] To investigate the effects of Shenqi compound on abnormal glucose and lipid metabolism，pathological process of skeletal muscle injury，and adiponectin signaling pathway in skeletal muscle of aging diabetes rats. [Methods] Ten 4-monthold male Wistar rats were included as control（C）group，ten 17-month-old male Wistar rats were included as aging model（SMo）group，and 30 17-month-old male Wistar rats were fed with high-fat and high-sugar diet and injected with STZ included as aging diabetes model. After modeling，they were randomly divided into aging diabetes model（STMo）group，traditional Chinese medicine（SQFF）group and Western medicine（X）group. They were combined with the former blank control（C）group and aging model（SMo）group to form five groups of rats. STMo group，SQFF group，and X group were fed with high-fat diet. Rats in group C and SMo were fed with regular feed. The SQFF group was given Shenqi compound freeze-dried powder by gavage，the X group was given sitagliptin suspension by gavage， and the C group，SMo group，and STMo group were given sodium chloride solution by gavage，with continuous intervention for 8 weeks. Detection indicators：Plasma APN，FBG，INS，TG，T-CHO，NEFA detection in each group of rats；HOMA-IR calculation；pathological morphology observation of gastrocnemius muscle under light and electron microscopy；qRT-PCR was used to detect the mRNA expression of AdipoR1，AdipoR2，AMPK，and PPAR-α；Western blot was used to detect the relative expression levels of AdipoR1， AdipoR2，AMPK，pAMPK，and PPARα proteins in gastrocnemius muscle tissue. [Results] Compared with group C，plasma FBG，TG，TCHO，NEFA，and HOMA-IR were significantly increased in the STMo group（P<0.05）；the mRNA expression of plasma APN， gastrocnemius muscle AdipoR1，AdipoR2，AMPK，PPARα，as well as the relative expression levels of AdipoR1，AdipoR2，AMPKα， pAMPKα，and PPARα proteins were significantly reduced（P<0.05）；compared with the STMo group，the plasma FBG，TG，T-CHO， and NEFA in the SQFF group and X group were significantly reduced（P<0.05），while the mRNA expression of plasma APN， gastrocnemius muscle AMPK，PPARα，and the relative expression levels of gastrocnemius muscle AdipoR1，AdipoR2，AMPKα， pAMPKα，and PPARα proteins were significantly increased（P<0.05）. In addition，compared with the STMo group，the HOMA-IR of X group was significantly reduced（P<0.05），while the SQFF group only showed a downward trend；compared with STMo，the mRNA expression of AdipoR2 in the SQFF group significantly increased（P<0.05），while the X group only showed an upward trend. The injury degree of gastrocnemius muscle under light microscope showed that Shenqi compound could improve the inflammation injury of skeletal muscle，muscle fibrosis，necrosis，fracture，dissolution，and proliferation of fiber tissue. Observation under electron microscope showed that Shenqi compound could improve the damage of skeletal muscle fiber structure and different degree of mitochondrial damage. [Conclusion] The Shenqi compound strengthens the spleen and promotes transportation. By restoring the spleen’s functions of transportation and transformation as well as muscle management，it improves the disorders of glucose and lipid metabolism and gastrocnemius muscle injury in aging diabetic rats. This may be related to the adiponectin signaling pathways AdipoR1/AMPK and AdipoR2/ PPARα. |
|
