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| Analysis of metabolic differentiators and pathways in patients with coronary artery disease with phlegm and blood stasis syndrome based on serum metabolomics |
| Hits 267 Download times 65 Received:September 28, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2026.02.03 |
| Key Words
metabolomics;coronary heart disease;phlegm and blood stasis;Chinese medicine |
| Author Name | Affiliation | E-mail | | WEN Tao | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SONG Zhihui | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Zhenzhen | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XIAO Yang | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | ZHAO Jia | Tianjin Chest Hospital, Tianjin 300222, China | orange2012@126.com | | WANG Yi | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangyi@tjutcm.edu.cn |
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| Abstract
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| [Objective]To apply metabolomics technology to analyze the metabolic differentiations and pathways in the serum of patients with coronary heart disease with phlegm and blood stasis syndrome, and to enrich the metabolomics study of coronary heart disease with phlegm and blood stasis syndrome.[Methods]A total of 62 patients with coronary heart disease in Tianjin Chest Hospital from March 2023 to July 2023 were collected and divided into 42 cases in the CHD-TYHJ group and 20 cases in the control group according to the disease and evidence type. The 2 mL of venous blood was drawn from the subjects on an empty stomach, and the serum was extracted after standing at room temperature for 1 hour. The levels of TC, TG, HDL-C and LDL-C in the sera of the two groups were detected by using fully automated biochemistry, followed by comparison of the metabolites in the sera of the two groups and signaling pathway enrichment analysis of the differential metabolites in the evidence of CHO-HYHJ by using the Metabo Analyst platform.[Results]Compared with the CHD-TYHJ group, serum HDL-C and LDL-C levels were reduced in the control group(P < 0.05), and there was no significant difference in TC and TG levels(P > 0.05).The metabolic profiles of the CHD-TYHJ group were well differentiated from those of the control group, and the OPLS-DA model was not overfitted, and the results shown were reliable. The metabolic profiles of the CHD-TYHJ group were better differentiated from those of the control group. Control group, a total of 82 metabolites were up-regulated and 19 metabolites were down-regulated in the positive ion model, and a total of 12 metabolites were up-regulated and 19 metabolites were down-regulated in the negative ion model. The four key pathways with the highest correlation with metabolite differences were, in order, glycerophospholipid metabolism, arachidonic acid metabolism, linoleic acid metabolism, and alpha-linolenic acid metabolism, linoleic acid metabolism, and alpha-linolenic acid metabolism.[Conclusion]Differential metabolites existed between CHD-TYHJ and control group, and the alpha-linolenic acid metabolic pathway was the first time to be found in the serum metabolomics of patients with coronary heart disease with phlegm and blood stasis syndrome. |
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