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| Exploring the effect of stigmasterol on autophagy of podocytes in rats with diabetic kidney disease based on the Sirt1/PGC-1α pathway |
| Hits 16 Download times 4 Received:December 08, 2025 |
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| DOI
10.11656/j.issn.1672-1519.2026.04.11 |
| Key Words
stigmasterol;Sirt1/PGC-1α pathway;diabetic kidney disease;podocytes;autophagy |
| Author Name | Affiliation | | HU Dongyi | Department of Nephrology, The Fifth Clinical College of Henan University of Chinese Medicine(People's Hospital of Zhengzhou), Zhengzhou 450000, China | | GU Dongfeng | Department of Nephrology, The Fifth Clinical College of Henan University of Chinese Medicine(People's Hospital of Zhengzhou), Zhengzhou 450000, China | | LIU Bianling | Department of Nephrology, The Fifth Clinical College of Henan University of Chinese Medicine(People's Hospital of Zhengzhou), Zhengzhou 450000, China | | ZHAO Jifang | Department of Nephrology, The Fifth Clinical College of Henan University of Chinese Medicine(People's Hospital of Zhengzhou), Zhengzhou 450000, China | | SHEN Beili | Department of Nephrology, The Fifth Clinical College of Henan University of Chinese Medicine(People's Hospital of Zhengzhou), Zhengzhou 450000, China | | WU Meng | Department of Nephrology, The Fifth Clinical College of Henan University of Chinese Medicine(People's Hospital of Zhengzhou), Zhengzhou 450000, China |
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| Abstract
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| [Objective] To discuss the effect of stigmasterol on autophagy of podocytes in diabetic kidney disease（DKD） rats based on the silent information regulator 1（Sirt1） / peroxisome proliferator-activated receptor γ coactivator 1α（PGC-1α） pathway. [Methods] DKD rats were established using high-glucose and high-fat，streptozotocin solution，and stochastically separated into the DKD group，different doses of stigmasterol（stigmasterol-low50 mg/kg，stigmasterol-high 200 mg/kg） groups，valsartan（8.33 mg/kg） group，and stigmasterol-high+EX527（5 mg/kg） group. And the rats fed with ordinary feed were regarded as the normal group. The urine protein content and blood glucose were detected. The levels of blood urea nitrogen（BUN） and serum creatinine（Scr） were detected by the kits. Renal tissues were isolated to evaluate histopathology and changes in podocyte structure and autophagosomes. The positive expressions of podocular pore membrane protein molecules（Nephrin，Podocin），the expressions of autophagy marker proteins（Beclin-1，P62） mRNA，and the expressions of Sirt1/PGC-1α pathway-related proteins in renal tissue were detected. [Results] The DKD group had lower positive expressions of Nephrin and Podocin，and the expressions of Beclin-1 mRNA，Sirt1，and PGC-1α proteins，and higher blood glucose，urine protein，Scr，BUN，and expression of P62 mRNA than the normal group（P<0.05）. The stigmasterol-low group，stigmasterol-high group and valsartan group had higher positive expressions of Nephrin and Podocin，and the expressions of Beclin-1 mRNA，Sirt1，and PGC-1α proteins，and lower blood glucose，urine protein，Scr，BUN，and expression of P62 mRNA than the DKD group（P<0.05）. The stigmasterol-high+EX527 group had lower positive expressions of Nephrin and Podocin，and the expressions of Beclin-1 mRNA，Sirt1，and PGC-1α proteins，and higher blood glucose，urine protein，Scr，BUN，and expression of P62 mRNA than the stigmasterol-high group（P<0.05）. [Conclusion] Stigmasterol enhances autophagy of podocytes in DKD rats and alleviates podocyte injury by activating the Sirt1/PGC-1α pathway. |
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