|
| Study on the mechanism of Luoheng Prescription in the prevention and treatment of coronary microvascular disease based on network pharmacology and experimental validation |
| Hits 55 Download times 2 Received:January 10, 2026 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1672-1519.2026.05.11 |
| Key Words
Luoheng Prescription;coronary microvascular disease;network pharmacology;molecular docking;inflammation |
| Author Name | Affiliation | E-mail | | WANG Wujiao | Beijing University of Chinese Medicine, Beijing 100029, China | | | LI Yuxuan | Beijing University of Chinese Medicine, Beijing 100029, China | | | LIU Bo | Beijing University of Chinese Medicine, Beijing 100029, China | | | WEI Xiaoqi | Beijing University of Chinese Medicine, Beijing 100029, China | | | WANG Xian | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100007, China | | | CHANG Peifen | Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100007, China | 13661022016@163.com | | LI Tianli | China-Japan Friendship Hospital, Beijing 100013, China | 1099188092@qq.com |
|
| Abstract
|
| [Objective] To explore the potential mechanism of Luoheng Prescription for the treatment of coronary artery microvascular disease（CMVD）. [Methods] 1） We analyzed the main components and targets for the treatment of CMVD of Luoheng Prescription through network pharmacology and used molecular docking to predict the binding activities of the core components and the key targets. 2） Thirty-two SD rats were randomly divided into the sham-operation group，the model group，the Luoheng Prescription group，and the Nicorandil group，with eight rats in each group. These rats were gavaged continuously for 2 weeks. Then，the coronary microvascular injury model was established by left ventricular injection of sodium laurate. The coronary flow reserve（CFR） was detected by ultra-high-resolution small animal ultrasonography，and myocardial pathological histomorphology was observed by hematoxylin-eosin staining. Transmission electron microscopy was used to observe microvascular endothelial cells and mitochondria. Enzyme-Linked Immunosorbent Assay（ELISA） was used to detect the levels of the inflammatory factors interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α）. [Results] 1）We obtained 44 active ingredients and 253 targets for the treatment of coronary artery microvascular disease by Luoheng Prescription；the top six core ingredients were isorhamnetin，Quercetin，Kaempferol，Jaranol，Linolenic acid，and Liquiritigenin；and the top six key targets were IL-6，TNF，signal transducer and activator of transcription 3（STAT3），Jun Proto-Oncogene（JUN），Serine/Threonine Kinase Proteins 1（AKT1），and tumor protein p53（TP53）；the molecular docking results show that the binding of the key active ingredients to the target targets is more stable. 2）The results of animal experiments showed that compared with the sham-operated group，the model group had significantly lower CFR（P<0.01） and higher levels of the inflammatory factors IL-6 and TNF-α（P<0.01）；extensive epicardial infiltration of lymphocytes and granulocytes，focal mesenchymal stasis，and necrosis of cardiomyocytes；and swelling of microvascular endothelium，mitochondria，and mitochondrial cristae lysis. Compared with the model group，CFR was significantly increased（P<0.01），and the levels of inflammatory factors IL-6 and TNF-α were significantly decreased（P<0.01） in the Luoheng Prescription group；lymphocyte and granulocyte infiltration，focal mesenchymal stasis，and necrosis of cardiomyocytes were ameliorated；and microvascular endothelial and mitochondrial cell swelling，and mitochondrial cristae lysis were all improved. [Conclusion] Luoheng Prescription can improve the coronary microvascular injury induced by sodium lauryl silicate and increase CFR. The mechanism may involve inhibiting the inflammatory response and reducing the swelling of endothelial cells and mitochondria by down-regulating the expression levels of IL-6 and TNF-α，thereby exerting cardioprotective effects. |
|
|
|
|
|
|
|
