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| Medicinal serum of Tougu Xuejie Powder inhibiting the malignant behavior of human rheumatoid arthritis synovial fibroblasts by blocking the PI3K/AKT signaling pathway |
| Hits 32 Download times 8 Received:February 05, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.05.14 |
| Key Words
Tougu Xuejie Powder;PI3K/AKT signaling pathway;rheumatoid arthritis;synovial fibroblasts;malignant behavior |
| Author Name | Affiliation | E-mail | | PENG Lili | Department of Integrated Traditional Chinese and Western Medicine, Changsha Third Hospital(Changsha Hospital Affiliated to Hunan University), Changsha 410015, China | | | DENG Wenwen | Department of Integrated Traditional Chinese and Western Medicine, Changsha Third Hospital(Changsha Hospital Affiliated to Hunan University), Changsha 410015, China | | | JIANG Ru | Department of Integrated Traditional Chinese and Western Medicine, Changsha Third Hospital(Changsha Hospital Affiliated to Hunan University), Changsha 410015, China | | | XIAO Zhi | Department of Integrated Traditional Chinese and Western Medicine, Changsha Third Hospital(Changsha Hospital Affiliated to Hunan University), Changsha 410015, China | xziiou@163.com |
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| Abstract
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| [Objective] To investigate whether the drug-containing serum of Tonggu Xuejie Powder can inhibit the malignant behavior of human rheumatoid arthritis synovial fibroblasts（RA-FLS） by blocking the phosphoinositide 3-kinase（PI3K）/protein kenase B（AKT） signaling pathway. [Methods] Human RA-FLS cells were cultured in vitro. The cells were divided into the following 4 groups：Group A（normal group），Group B [model group，induced inflammatory microenvironment by lipopolysaccharide（LPS）]，Group C（LPS+Tougu Xuejie Powder group），and Group D（LPS+Tougu Xuejie Powder+PI3K activator group）. Cell viability was detected by CCK-8，apoptosis was detected by flow cytometry，cell migration was detected by scratch assay，cell invasion was detected by Transwell，and the levels of inflammatory factors in cell supernatants[Tumor necrosis factor-α（TNF-α），interleukin-1β（IL-1β），interleukin-6（IL-6），interleukin-10（IL-10）] were detected by Enzyme-linked immunosorbent assay（ELISA），and the expression levels of PI3K/AKT signaling pathway-related proteins were detected by western blot. [Results] The CCK8 experiment results showed that 15% and 20% of the drug-containing serum of Tougu Xuejie Powder significantly reduced the cell viability of RA-FLS（both P<0.05） at 24 hours and 48 hours of intervention. Compared with group A，the cell proliferation，migration，invasion quantity，and levels of TNF-α，IL-1β，and IL-6 in the supernatant of cells in group B were significantly increased，while the apoptosis rate and the level of IL-10 in the supernatant of cells decreased，and the ratios of p-PI3K/PI3K，pAKT/AKT，p-mTOR/mTOR and p-GSK-3β/GSK-3β increased（all P<0.05）. This indicates that LPS stimulation promotes the malignant behavior of RA-FLS cells，activates the PI3K/AKT signaling pathway，and enhances the phosphorylation levels of downstream key molecules（such as mTOR and GSK-3β）.After 24 hours of treatment with the drug-containing serum of Tougu Xuejie Powder，the proliferation，migration，invasion quantity，levels of TNF-α，IL-1β，and IL-6 in the supernatant of RA-FLS cells，and the ratios of p-PI3K/PI3K，pAKT/AKT，p-mTOR/mTOR and p-GSK-3β/GSK-3β decreased，while the apoptosis rate and the level of IL-10 in the supernatant of cells increased. Activation of the PI3K/AKT signaling partially reversed the inhibitory effect of the drug-containing serum of Tougu Xuejie Powder on the proliferation，migration，and invasion abilities of RA-FLS cells，as well as the improvement effect on the inflammatory response of RA-FLS cells. [Conclusion] The medicinal serum of Tougu Xuejie Powder inhibits the malignant behavior of human rheumatoid arthritis synovial fibroblasts by blocking the PI3K/AKT signaling pathway. |
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