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| Identification of in vivo components and exploration of the mechanism of action of Qishen Yiqi Dripping Pills based on UPLC-Q-TOF-MS/MS |
| Hits 240 Download times 57 Received:January 05, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.06.11 |
| Key Words
Qishen Yiqi Dripping Pills;UPLC-Q-TOF-MS/MS;in vivo component identification;myocardial energy metabolism |
| Author Name | Affiliation | E-mail | | LI Bin | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | DU Kunze | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, National Key Laboratory of Modern Chinese Medicine Creation, Tianjin 301617, China | | | YANG Man | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Qian | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Shuo | Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, National Key Laboratory of Modern Chinese Medicine Creation, Tianjin 301617, China | | | WANG Yanguo | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | LIU Yijia | Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | lyj1204566353@163.com | | REN Ming | Baokang Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China | renming2008@126.com |
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| Abstract
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| [Objective] This study employed ultra- performance liquid chromatography coupled with quadrupole time- of-flight mass spectrometry（UPLC-Q-TOF-MS/MS）to systematically identify the absorbed constituents of Qishen Yiqi Dripping Pills（QSYQ）in rat serum and myocardial tissue，and to elucidate the potential cardioprotective mechanisms through energy metabolism-related signaling pathways. [Methods] UPLC-Q-TOF-MS/MS was used to analyze serum and myocardial tissue samples from rats administered QSYQ. The chemical structures of the detected compounds were confirmed through high-resolution natural product databases and literature comparison. Based on recent literature on myocardial energy metabolism，the study explored the energy-regulating effects of the absorbed constituents，focusing on pathways related to energy substrate utilization，mitochondrial function，purine cycling，and redox homeostasis. [Results] A total of 12 compounds were identified in serum and 9 in myocardial tissue，primarily including 11 amino acids，2 fatty acids，3 phenolic acids，1 flavonoid，2 purine derivatives，1 amide，and 1 heptane. [Conclusion] QSYQ may exert its cardioprotective effects through a“multi-component，multi-pathway，and multi-target”synergistic regulation of key energy metabolism networks，such as AMPK-mTOR-SIRT1，AMPK-Nrf2-PINK1/Parkin，TCA anaplerosis，and Nrf2-NF-κB pathways，thereby improving myocardial energy imbalance. This provides experimental evidence supporting the material basis for its pharmacological effects. |
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