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| Prescription-splitting study on Simiao Yong’an Decoction in reducing atherosclerotic inflammatory factor high sensitivity C-reactive protein |
| Hits 214 Download times 59 Received:January 21, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.06.13 |
| Key Words
Simiao Yong’an Decoction;honeysuckle;atherosclerosis;inflammation;high sensitivity C-reactive protein |
| Author Name | Affiliation | E-mail | | SU Wenquan | Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China | | | FAN Qinhua | Department of Traditional Chinese Medicine, Peking University People's Hospital, Beijing 100044, China | | | DU Yawei | National Drug Clinical Trial Institution, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | | | WU Shengxian | National Drug Clinical Trial Institution, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China | wushx@sina.com |
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| Abstract
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| [Objective] To identify the core component of Simiao Yong’an Decoction responsible for reducing high sensitivity C-reactive protein（hs-CRP），a key inflammatory factor in atherosclerosis，and provide experimental evidence for precise clinical medication. [Methods] Single-herb disassembling experiments were performed. An inflammation model was established by feeding a high-fat diet combined with injections of lipopolysaccharide（LPS）and vitamin D3. Thirty Sprague-Dawley（SD）rats were randomly divided into 6 groups（5 rats per group）：blank control group，model group，Lonicera japonica group，Angelica sinensis group，Scrophularia ningpoensis group，and Glycyrrhiza uralensis group. Serum hs-CRP levels in rats were dynamically monitored after drug intervention. To double-verify the core herb’s regulatory effect on CRP，two inflammation models were constructed：a conditioned medium co-culture model of RAW264.7 macrophages and HepG2 hepatocytes，and a direct co-culture inflammation model. An atherosclerosis model was established via right common carotid artery cannulation combined with high-fat diet feeding. Twenty ApoE-/- mice were randomly assigned to 4 groups（5 mice per group）：model group，statin group，colchicine group，and Lonicera japonica group. C57BL/6J mice fed a regular diet served as the normal control group. The potential mechanism of the core herb was explored. [Results] In the herb-disassembling animal experiment：Compared with the blank control group，the model group exhibited a significant increase in hs-CRP levels（P<0.05 or P<0.01）. Relative to the model group，the Lonicera japonica group showed a continuous decrease in hs-CRP levels on days 4，7，and 10（presenting a time-dependent effect），with a significantly lower level than the model group on day 10（P<0.05）. No significant differences were observed in the Angelica sinensis，Scrophularia ningpoensis，or Glycyrrhiza uralensis groups（P>0.05）. In the transfer and co-culture cell models：Both the ethanol extract and aqueous extract of Lonicera japonica significantly reduced CRP levels（P<0.01），and their effects were comparable to those of the positive control（P>0.05）. Mechanistic studies：Compared with the model group，the Lonicera japonica group showed markedly decreased expression levels of NF-κB（P<0.001），IL-1β（P<0.001），and IL- 6（P<0.001）；its effects were similar to those of the colchicine group（P>0.05）. [Conclusion] Lonicera japonica is the key herb in Simiao Yong’an Decoction for reducing the inflammatory factor hs-CRP in atherosclerosis，and its action is associated with the inhibition of the NF-κB/IL-1β/IL-6/CRP pathway. This study provides directions for the clinical application of traditional Chinese medicine and drug development in anti-inflammatory therapy for atherosclerosis. |
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