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| Effect of phillyrin on H2O2-induced cardiomyocyte damage by regulating the SphK1/S1P/S1PR1 pathway |
| Hits 78 Download times 30 Received:March 28, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.07.10 |
| Key Words
phillyrin;SphK1/S1P/S1PR1 pathway;H2O2;cardiomyocyte damage |
| Author Name | Affiliation | | WANG Yuqian | Department of Cardiology Ⅰ, Tangshan Hospital of Traditional Chinese Medicine, Tangshan 063000, China | | ZHANG Yan | Department of Cardiology Ⅰ, Tangshan Hospital of Traditional Chinese Medicine, Tangshan 063000, China | | ZHANG Weili | Department of Cardiology Ⅰ, Tangshan Hospital of Traditional Chinese Medicine, Tangshan 063000, China |
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| Abstract
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| [Objective] To investigate the effect of phillyrin（Phi）regulating the sphingosine kinase 1（SphK1）/sphingosine 1 phosphate （S1P）/sphingosine 1 phosphate receptor 1（S1PR1）pathway on H2O2-induced cardiomyocyte damage. [Methods] The oxidative damage model of H9c2 cardiomyocytes induced by H2O2 was adopted. H9c2 cells and H2O2-induced H9c2 cells were treated with different concentrations of Phi respectively. The viability of cells in each group was detected by CCK-8，and the treatment concentration of Phi was screened. The H 2O2-induced H9c2 cells were randomly divided into H2O2 group，low-dose phillyrin group（L-Phi group）， medium-dose phillyrin group（M-Phi group），high-dose phillyrin group（H-Phi group），high-dose phillyrin+pcDNA-NC group（H-Phi+ NC group），and high-dose phillyrin+pcDNA-SphK1 group（H-Phi+SphK1 group）. The proliferation ability of cells in each group was detected by EDU. Apoptosis of cells in each group was detected by flow cytometry. ELISA kits were used to detect the release of lactate dehydrogenase（LDH）and the levels of oxidative stress-related factors. The DCFH-DA fluorescence method was used to detect the level of mitochondrial reactive oxygen species（ROS）. The change in mitochondrial membrane potential（MMP）was detected by JC-1 method. In addition，Western blot was used to detect the proliferation，apoptosis of cells in each group and the expression of proteins related to the SphK1/S1P/S1PR1 pathway. [Results] Phi had no effect on the viability of H9c2 cells（P>0.05）. With the increase of Phi concentration，the viability of H9c2 cardiomyocytes induced by H2O2 increased（P<0.05）. Cells in the L-PHI group，M-Phi group and H -Phi group were treated with 5，10 and 20 μmol/L respectively. Compared with the NC group，the H2O2 group had lower rate of EDU positive cells，expressions of SOD，catalase（CAT），MMP，and CyclinD1，and higher apoptosis rate，LDH，MDA，ROS fluorescence intensity，expressions of Bax，Cleaved Caspase-9，SphK1，S1P，and S1PR1（P<0.05）. Compared with the H2O2 group，the L-Phi group， M-Phi group and H-Phi group had higher rate of EDU positive cells，expressions of SOD，CAT，MMP，and CyclinD1，and lower apoptosis rate，LDH，MDA，ROS fluorescence intensity，expressions of Bax，Cleaved Caspase-9，SphK1，S1P，and S1PR1（P<0.05）. Compared with the H-Phi group and H-Phi+NC group，the H-Phi+SphK1 group had lower rate of EDU positive cells，expressions of SOD，CAT，MMP，and CyclinD1，and higher apoptosis rate，LDH，MDA，ROS fluorescence intensity，expressions of Bax，Cleaved Caspase-9，SphK1，S1P，and S1PR1（P<0.05）. [Conclusion] Phi can alleviate H2O2-induced cardiomyocyte damage，which may be related to the inhibition of the activation of the SphK1/S1P/S1PR1 pathway. |
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