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| Exploration on the mechanism of Xuanfei Qingluo Formula on mycoplasma pneumoniae-induced paroxysmal cough model rats based on the TLR4/MyD88/NF-κB pathway |
| Hits 56 Download times 35 Received:March 16, 2026 |
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| DOI
10.11656/j.issn.1672-1519.2026.07.12 |
| Key Words
Xuanfei Qingluo Formula;TLR4/MyD88/NF-κB pathway;paroxysmal cough |
| Author Name | Affiliation | E-mail | | LI Yadong | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | ZHANG Qi | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | LIU Weiwei | The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300250, China | | | SUN Yuechen | Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China | 635208249@qq.com |
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| Abstract
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| [Objective] To explore the effect and mechanism of Xuanfei Qingluo Formula in the treatment of paroxysmal cough caused by Mycoplasma pneumoniae pneumonia（MPP）. [Methods] Sixty Wistar rats were randomly divided into a blank group，a mycoplasma pneumonia group，a mycoplasma pneumonia with paroxysmal cough group，a traditional Chinese medicine group，a Western medicine group，and a combined traditional Chinese and Western medicine group，with 10 rats in each group. Except for the blank group，the mycoplasma pneumonia group was intranasally instilled with mycoplasma pneumonia culture fluid（1×107 CCU/mL）to establish a MPP pneumonia model，while the other groups were intranasally instilled with mycoplasma pneumonia culture fluid（1×107 CCU/mL）and exposed to smoke and capsaicin solution nebulization to establish a mycoplasma pneumonia-induced paroxysmal cough rat mode. After successful modeling，the traditional Chinese medicine group was given 14 g/（kg·d），the Western medicine group was given 63 mg/（kg·d）， and the combined traditional Chinese and Western medicine group was given 14 g/（kg·d）+ 63 mg/（kg·d）of the corresponding drug solution by gavage for 7 consecutive days. The blank group，the mycoplasma pneumonia group，and the mycoplasma pneumonia with paroxysmal cough group were given 0.9% sodium chloride solution by gavage as a control. 24 hours after the last administration，the levels of interleukin-6（IL-6），interleukin-8（IL-8），and tumor necrosis factor-α（TNF-α）in the rat serum were detected by enzyme-linked immunosorbent assay（ELISA）. The expression levels of Toll-like receptor 4（TLR4），myeloid differentiation factor 88（MyD88）， and nuclear factor-κB（NF-κB）in the lung tissue of rats were detected by Western blot，and the mRNA expression levels of IL-6，IL-8， TNF-α，TLR4，MyD88，and NF-κB were detected by real-time fluorescence quantitative polymerase chain reaction（Real-time PCR）. [Results] Compared with the blank group，the levels of IL-6，IL-8 and TNF-α in the serum of rats in the mycoplasma pneumonia group and the mycoplasma pneumonia with paroxysmal cough group were significantly increased（P<0.05 or P<0.001）；the expression levels of TLR4，MyD88 and NF-κB proteins in lung tissue were significantly upregulated（P<0.001 or P<0.01）；the expression levels of TLR4，MyD88，NF-κB，IL-6，TNF-α and IL-8 mRNA in lung tissue were significantly upregulated（P<0.001 or P<0.01）. After treatment，compared with the mycoplasma pneumonia with paroxysmal cough group，the levels of IL-6，IL-8 and TNF-α in the serum of rats in the traditional Chinese medicine group，the Western medicine group and the integrated traditional Chinese and Western medicine group were significantly decreased（P<0.01 or P<0.001）；the expression levels of TLR4，MyD88 and NF-κB proteins in lung tissue were significantly decreased（P<0.01 or P<0.001）；the expression levels of TLR4，MyD88，NF-κB-p65，IL-6，IL-8 and TNF-α mRNA in lung tissue were significantly decreased（P<0.001）. [Conclusion] The Xuanfei Qingluo Formula can exert therapeutic effects on mycoplasma pneumonia-induced spasmodic cough by regulating the TLR4/MyD88/NF-κB signaling pathway，inhibiting the oxidative stress caused by MPP，and reducing airway hyperresponsiveness，thereby improving the microcirculation disorder of lung tissue. |
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